ISSN:
1612-1112
Keywords:
Membrane chromatography
;
Kistler and Nitschmann's fractionation
;
Depth filtration
;
Blood albumin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The aim of this work was to recover albumin from Kistler and Nitschmann's fraction IV using membrane chromatography. The best solubilization results were obtained at pH 6.5 using a phosphate buffer containing 150 mM NaCl. More than 90% of the initial albumin content was recovered. The purification procedure included 2 main steps: the first one was a depth filtration in order to remove fine particles and a selective depth filter treatment for lipid removal. The second step was ion exchange chromatography. We used membrane chromatography systems where the fluid flows radially allowing fast flow purification under low operating pressures. The eluted albumin was free from IgG. Because of the absence of contaminating IgG and its high microbiological quality compared to standard animal sera, this albumin preparation can be used as a culture medium additive. It can also be further purified by ultrafiltration. The equipment used here is easy to handle and to sterilize, and meets the FDA Code of Federal Regulations. Additionally, this procedure is flexible enough to allow the co-purification of other fraction IV proteins such as transferrin or alpha-1-anti-trypsin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02262462
Permalink