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  • 1
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Journal of Chemical Technology AND Biotechnology 69 (1997), S. 77-85 
    ISSN: 0268-2575
    Keywords: Penicillium citrinum ; lipases ; purification ; AOT reversed micelles ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: ---This work describes the extraction and back-extraction of a lipase from crude extract of Penicillium citrinum using AOT reversed micelles in isooctane. The effect of pH, ionic strength, AOT concentration on the protein forward and backward transfer at 20°C was studied. The maximum protein forward extraction (32·0%) was achieved at pH 4·0 with a 50 mmol dm-3 acetate buffer containing 100 mmol dm-3 KCl and 100 mmol dm-3 AOT in isooctane. Proteins were back-extracted (82·7%) to a new aqueous phase containing 100 mmol dm-3 pH 8·0 phosphate buffer and 1000 mmol dm-3 KCl. No enzyme activity could be detected either in the micellar phase or in the aqueous phase after protein back-extraction. However, the lipolytic activity was recovered after hydrophobic interaction chromatography on a Phenyl Superose column. The yield obtained for the overall process was 68% for activity, 26·4% for protein recovery and the purification factor was 810-fold. A single protein band at 33000 Da was obtained for SDS-PAGE analysis for the recovered and purified enzyme. © 1997 SCI.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Journal of Chemical Technology AND Biotechnology 73 (1998), S. 377-384 
    ISSN: 0268-2575
    Keywords: zeolite ; ethanol fermentation ; Saccharomyces bayanus ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zeolite NaY at 5 g dm-3 concentration, was selected to improve the production of ethanol fermentation by Saccharomyces bayanus from high glucose concentration media. The highest ethanol productivity (3·07 g dm-3 h-1) was obtained from a 220 g dm-3 initial glucose concentration, while the highest ethanol concentration (130 g dm-3) was obtained from a 350 g dm-3 glucose medium. The zeolite is believed to have acted as a pH regulator, maintaining the pH value around 3·7-3·8. Under these conditions cellular viability was preserved and metabolic activity was maintained. Thus all the glucose was consumed, and high ethanol productivity and concentration were obtained. Therefore, the addition of zeolite improved ethanol production from high concentrations of glucose by Saccharomyces bayanus. © 1998 Society of Chemical Industry
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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