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  • 1
    Electronic Resource
    Electronic Resource
    Transformation of Phanerochaete chrysosporium and Neurospora crassa with adenine biosynthetic genes from Schizophyllum commune (1990)
    Springer
    Current genetics 17 (1990), S. 305-311 
    Details
    ISSN: 1432-0983
    Keywords: Phanerochaete chrysosporium ; Transformation ; Heterologous expression ; Basidiomycetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasted basidiospores of two different adenine auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium were transformed to prototrophy using plasmids containing genes encoding adenine biosynthetic enzymes from Schizophyllum commune. Fragments containing these genes were subcloned into pUC18 and P. chrysosporium transformants obtained with these subclones were analyzed. The subclones were mapped for restriction sites and the approximate locations of the complementing genes were determined. One of these plasmids was used to transform the Neurospora crassa auxotrophic strain ade2, thereby identifying the S. commune ade5 biosynthetic gene as encoding phosphoribosylaminoimidazole synthetase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00314877
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Homologous transformation of the lignin-degrading basidiomycete Phanerochaete chrysosporium (1991)
    Springer
    Current genetics 19 (1991), S. 491-494 
    Details
    ISSN: 1432-0983
    Keywords: Phanerochaete chrysosporium ; DNA transformation ; Basidiomycete ; Adenine biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A clone containing the Phanerochaete chrysosporium ade1 gene was isolated from a λEMBL3 genomic library using the ade5 gene encoding aminoimidazole ribonucleotide synthetase, from Schizophyllum commune, as a probe. A 6.0 kb fragment incorporating the ade1 gene was subcloned into pUC18 (pADE1) and used to transform the P. chrysosporium ade1 auxotrophic strain. Transformation frequencies were similar to those obtained previously with the S. commune ade5 gene; however, homologous transformants arose earlier than heterologous transformants. The transformants were mitotically and meiotically stable and Southern blot analysis indicated that the plasmid, pADE1, integrated ectopically in single or multiple copies. The pADE1 insert was mapped for restriction sites and the approximate location of the ade1 gene within the insert was determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312741
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium (1993)
    Springer
    Current genetics 23 (1993), S. 351-356 
    Details
    ISSN: 1432-0983
    Keywords: Phanerochaete chrysosporium ; DNA transformation ; Basidiomycete ; Uracil auxotrophs ; Homothallism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Uracil auxotrophs of Phanerochaete chrysosporium were isolated using 5-fluoroorotate resistance as a selection scheme. The ura3 auxotrophs deficient in orotidylate decarboxylase and ura5 auxotrophs deficient in orotate phosphoribosyl transferase were characterized by enzyme assays and complementation tests. The ura5 auxotrophs were transformed to prototrophy with the ura5 gene from the ascomycete Podospora anserina. The ura3 auxotrophs were transformed to prototrophy with the ura3 gene from the basidiomycete Schizophyllum commune. The P. chrysosporium ura3 gene was isolated from a γEMBL3 genomic library using the S. commune ura3 gene as a probe. A 6.6-kb fragment incorporating the ura3 gene was subcloned into Bluescript SK+(pURA3.1) and used to transform P. chrysosporium ura3 auxotrophic strains. The pURA3.1 insert was mapped for restriction sites and the approximate location of the ura3 gene within the insert was determined. Double auxotrophic strains were transformed with either of two marker genes and the resulting single auxotrophic strains were crossed to demonstrate genetic recombination between two nuclei of identical genetic background.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310898
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  • 4
    Electronic Resource
    Electronic Resource
    Truncated-gene reporter system for studying the regulation of manganese peroxidase expression (1997)
    Springer
    Current genetics 31 (1997), S. 519-524 
    Details
    ISSN: 1432-0983
    Keywords: Key words Manganese peroxidase ; Lignin degradation ; Phanerochaete chrysosporium ; Gene regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The expression of manganese peroxidase (MnP) in nitrogen-limited cultures of Phanerochaete chrysosporium is regulated by Mn, heat shock (HS), and H2O2 at the level of gene transcription. We have constructed a homologous gene reporter system to further examine the regulation of two mnp genes, mnp1 and mnp2, encoding individual MnP isozymes. Internal deletions of 234 and 359 bp were made within the coding regions of the mnp1 and mnp2 genes, respectively. The truncated mnp genes were subcloned into the shuttle vector pOGI18, which includes the Schizophylum commune ade5 gene as a selectable marker, and transformed into a P. chrysosporium Ade1 auxotrophic mutant. Northern-blot analysis of purified Ade+ transformants demonstrated that both of the truncated mnp genes were regulated in a manner similar to the endogenous mnp genes with respect to nitrogen limitation and induction by Mn, HS, and H2O2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050239
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    Paper (German National Licenses)
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