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  • 1
    Electronic Resource
    Electronic Resource
    Transcription of the groESL operon in Pseudomonas aeruginosa PAO1 (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 163 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Northern hybridization and S1 nuclease mapping demonstrated that the groES and groEL genes in Pseudomonas aeruginosa were transcribed as a bicistronic mRNA of 2.2 kb. Two transcription start sites and a transcription termination site were mapped. Overlapping consensus sequences for σ32- and σ70-dependent promoters were found in the upstream region of groES. Levels of groESL-specific mRNA were increased about 2-fold upon heat shock. This response differs from the dramatic enhancement (more than 10-fold) of groESL transcription after heat shock observed in other bacterial species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13051.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Heterologous expression of the cytochrome P450cam hydroxylase operon and the repressor gene of Pseudomonas putida in Escherichia coli (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 123 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The cytochrome P450cam hydroxylase operon (camDCAB) of Pseudomonas putida is negatively regulated by a repressor, CamR, which also represses its own gene. The expression in P. putida of both camR and camDCAB is depressed in the presence of d-camphor. We examined the expression in Escherichia coli of camR and camDCAB by monitoring the enzyme activity of the camD gene product. In the presence or absence of d-camphor in the cell culture, the expression in E. coli of camD was significant and constitutive, suggesting no expression of camR. This lack of expression was confirmed by monitoring the β-galactosidase activity of camR-lacZ translational fusions. However, S1 nuclease mapping revealed that synthesis of camR mRNA in E. coli was significant and constitutive, as observed in the case of camDCAB mRNA. Thus, it is likely that the expression of camR in E. coli is limited at the translational level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07200.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Studies on cyclic β-1,2-glucan obtained from periplasmic space ofRhizobium trifolii cells (1982)
    Springer
    Plant and soil 64 (1982), S. 315-324 
    Details
    ISSN: 1573-5036
    Keywords: Bacterial cell deformation ; Cyclic β-1,2-glucan ; Infection ; Nodule ; Periplasmic space ; Rhizobium trifollii ; White clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A glycan was obtained from the periplasmic space ofRhizobium trifolii cells by saccharose-osmotic shock method. This glycan was composed of only β-1,2-linked glucose, having no end groups (reducing and non-reducing) and branching points. It was concluded that this glucan has a structure of unbranched cyclic form. When the cyclic β-1,2-glucan was added to the seedlings of white clover as a host plant withR. trifolii, it promoted not only the infection thread formation but also the nodule formation. In the culture medium containing the cyclic β-1,2-glucan, cells were found agglutinated during the middle logarithmic phase of growth, and their morphological changes were recognized as cell elongation or swelling in the middle region of a cell when observed in scanning electron microscope (SEM).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02372514
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    Paper (German National Licenses)
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