ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
An acid phosphatase (EC 3.1.3.2.) from the embryonic axes of chickpea seeds (Cicer arietinum L. cv. Castellana) was purified by ammonium sulphate precipitation, chromatography on Sephacryl S-200 and polyacrylamide gel electrophoresis. The preparation has an apparent molecular weight of 39 kDa, pH optimum for p-nitrophenylphosphate hydrolysis of 5.25, and Km of 0.57 mM. The enzyme hydrolyzed all the mono- and di-phosphorylated sugars tested, but had no effect on ATP, ADP, AMP and phosphoenolpyruvate. Phosphate was a competitive inhibitor. Mg2+. Ca2+, Hg2+, Fe3+, arsenate, K+ and Zn2+ were inhibitory. Mn2+, dithiothreitol and EDTA had no effect, and polyamines were activators.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1988.tb02042.x
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