ISSN:
1573-7381
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The several methods that have been used to count synapses by electron microscopy are put in error by the inevitable failure to recognize and count all of the smaller profiles of synapses present in a section. The smaller profiles are produced by near-tangential sectioning of synapses of larger diameter, whereas equatorial sections produce large profiles that are always recognized. The expected number of smaller profiles can be calculated from the observed numbers of larger profiles by the method of Coupland (1968). The estimated density is then dependent on the more reliable observations only, but the iterative calculation required is very tedious. This paper describes the calculation (performed by a computer), and discusses the methods available for counting synapses, and the results obtained.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01097194
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