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  • 1
    Electronic Resource
    Electronic Resource
    Evidence of a strong, positive association between atopy and the HLA class II alleles DR4 and DR7 (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 26 (1996), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Atopy, with or without associated asthma, provides a useful model for evaluating the genetic factors that control human immune responsiveness. HLA class II gene products are involved in the control of immune responses.Objectives We investigated whether susceptibility or resistance to the disease was associated with HLA class II genes.Methods Blood samples were obtained from two groups of unrelated European-born white adults: 56 atopic patients (52 of them with asthma) and 39 healthy controls with no personal or familial history of asthma or atopy. Genomic DNA was extracted from peripheral blood lymphocytes. The exons of DQA1, DQB1, DRB and DPBl genes were selectively amplified by the polymerase chain reaction (PCR) method. Geno-typing was carried out by digestion of the amplified DNA products with allele-specific endonucleases (PCR-RFLP), which can recognize allelic variations in the polymorphic exon.Results We found no significant difTerences in the frequency of DPBl alleles between patients and controls. HLA class II DR4 and DR7 alleles were present in 39.2% of the patients and in 2.5% of the healthy subjects (Pc*2± 3.9 10−3). Conversely, DQA1*0103 and DQB1*0502 alleles were more frequent in the control subjects. These results confirm a previous study of an extended pedigree, which showed that DR4 and DR7 alleles were absent in all healthy members of the family and were frequently observed in atopic and/or in asthmatic subjects.Conclusion We observed that HLA-DR 4 and DR7 alleles are significantly implicated in their susceptibility to the disease and suggest that this susceptibility is more related to atopy than to specific responses to allergens. According to previous studies, we could also submit that in atopic patients with asthma, DR4 alleles at the least, could be more closely associated with atopy than with asthma per se. Conversely, we suggest that some allelic DQA1 and DQB1 sequences might confer protection against the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1996.tb00614.x
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  • 2
    Electronic Resource
    Electronic Resource
    HLA class II haplotype in atopic asthmatic and non-atopic control subjects (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 25 (1995), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1995.tb00425.x
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  • 3
    Electronic Resource
    Electronic Resource
    From Landscape to Individuality (1995)
    Cambridge : Cambridge University Press
    Archaeological dialogues 2 (1995), S. 28-30 
    Details
    ISSN: 1380-2038
    Source: Cambridge Journals Digital Archives
    Topics: Archaeology
    Notes: Several years ago, when I visited the Netherlands for the first time, a famous historian with whom I was walking along a road through the polders observed that the national character of the Dutch had formed through many centuries of efforts to shape nature, and that nature in its turn bears the imprint of their character. I was greatly interested by this remark on the interaction between mentality and landscape and I asked my colleague to write an article on this subject for the periodical Odissei. Chelovek v istorii (‘Odysseus. Man in history’), which focused on issues of historical anthropology and which I am publishing for several years. Although unfortunately I did not receive the article as such, I was all the more interested to read Nico Roymans' work. The perception of space and landscape does in truth change through history and it would be incorrect to interpret the natural environment as a rigid framework in which the history of mankind unfolds. It is said that culture is man's second nature – but would it not be nearer the truth to say that it is his only nature? Man is a symbolical being (animal symbolicum), who finds his way in this world by means of symbols and who perceives reality through these points of reference, which he creates or reproduces. There is no sphere of activity beyond the boundary of this symbolical world. For this reason the perception of human activity cannot go beyond or neglect this all-embracing symbolical universe. Nevertheless far from all historians have mastered the art of reading or deciphering the sign systems of the past or present, since it is by no means easy to learn to read in this manner.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1017/S1380203800000283
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  • 4
    Electronic Resource
    Electronic Resource
    Differentiation under the control of insulin of rat preadipocytes in primary culture - Isolation of homogeneous cellular fractions by gradient centrifugation
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 762 (1983), S. 437-444 
    Details
    ISSN: 0167-4889
    Keywords: Cell separation ; Density gradient centrifugation ; Differentiation control ; Insulin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4889(83)90009-5
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  • 5
    Electronic Resource
    Electronic Resource
    Development under the control of insulin of lipogenic enzymes, lipoprotein lipase, isoproterenol and glucagon sensitivity in differentiating rat preadipocytes in primary culture
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 805 (1984), S. 252-260 
    Details
    ISSN: 0167-4889
    Keywords: (Rat preadipocyte) ; Differentiation ; Glucagon ; Hormone sensitivity ; Insulin ; Isoproterenol ; Lipogenic enzyme ; Lipoprotein lipase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4889(84)90080-6
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  • 6
    Electronic Resource
    Electronic Resource
    A simple and rapid micromethod for genomic DNA extraction from jugal epithelial cells (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 49 (1994), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We describe a rapid and reliable micromethod for DNA isolation from buccal epithelial cells from the interior mouth mucosa. This convenient, noninvasive method could be applied to genetic typing in a small number of cells (about 2000 cells per cheek). We have shown that DNA released by this method is suitable for further amplification by polymerase chain reaction (PCR). Using this protocol, coupled with the PCR-RFLP (restriction fragment length polymorphism) method, we analyzed the allelic sequence diversity of the human lymphocyte antigen (HLA) class II genes in an extended family of 33 persons containing 14 asthmatic or atopic members. Six of eight DQA1 alleles, and 11 DQB1, 20 DPB1, and 10 DR haplotypes could be identified in a single DNA sample. Our results suggest that the DR53 group haplotype is frequently associated with allergic asthma and atopy. The micromethod described here may be useful in genetic epidemiology, especially in family studies involving small children.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1994.tb02105.x
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  • 7
    Electronic Resource
    Electronic Resource
    Analysis of hsp70 gene polymorphism in allergic asthma (1999)
    Copenhagen : Munksgaard International Publishers
    Allergy 54 (1999), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Allergic asthma is a multifactorial and probably multigenic inflammatory disease of the upper airways, and has been associated with the HLA class II alleles DR4 and DR7. Here we investigated possible associations with other polymorphic susceptibility/resistance genes located within the major histocompatibility complex, i.e., the genes coding the major 70-kDa heat-shock proteins (HSP; Hsp70) hsp70–1, hsp70–2, and hsp70-HOM, whose products are overexpressed in the bronchi of asthmatic patients. Methods: Genomic DNA was extracted from peripheral blood lymphocytes or buccal epithelial cells of 48 patients with allergic asthma and 31 selected nonatopic control subjects, in whom we previously reported a strong association of atopy with DR4/DR7 alleles.Results: No evidence was found for an independent role of hsp70 gene polymorphism in susceptibility to allergic asthma. However, hsp70 alleles might be involved in extended haplotypes of HLA markers. Conclusions: Our data suggest that Hsp70 overexpression in asthma results from complex interactions between environmental exposures and genetic background rather than from specific genetic variations in hsp70 genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.1999.00859.x
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  • 8
    Electronic Resource
    Electronic Resource
    Chloroplast DNA variation and evolution in the genus Lens Mill (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 452-457 
    Details
    ISSN: 1432-2242
    Keywords: Key words Lentil ; Evolution ; Domestication ; cpDNA ; Plastid inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Chloroplast DNA (cpDNA) restriction site diversity was assessed by 21 enzyme/probe combinations in 30 accessions of six Lens species, including the recently recognized L. lamottei and L. tomentosus. A total of 118 fragments were scored and 26 restriction site mutations were identified. The cpDNA restriction pattern supports circumscribing L. lamottei and L. tomentosus as independent species. The value of the data for reconstructing phylogeny in the genus is discussed. The cpDNA of all 13 accessions of the lentil’s wild progenitor, L. culinaris subsp. orientalis, differed from that of the single lentil cultivars used in this study. This diversity indicates that other populations of this subspecies from Turkey and Syria examined by Mayer and Soltis (1994) are potentially the founder members of lentil. Examination of L. lamottei×L. nigricans hybrids between accessions having different restriction patterns showed paternal plastid inheritance in L. nigricans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050436
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