ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Isoelectric focusing in mixed carrier ampholyte-immobilized pH gradients (CA-IPG) is an effective way to separate proteins by charge. A method to prepare CA-IPG in capillary tubes and the description of the equipment used are outlined. Two-dimensional gel electrophoresis patterns of human serum and liver biopsies, with isoelectric focusing and CA-IPG as the first dimension, are presented. The comparison with two-dimensional gel electrophoresis patterns obtained by conventional carrier ampholyte pH gradient separation shows the excellent resolution and potential of this technique.
Additional Material:
11 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150071105
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