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  • 1
    ISSN: 1573-2932
    Keywords: enteroviruses ; reoviruses ; seawaters ; estuarine waters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract 63 samples (53 seawater and 10 estuarine water samples) of 20 L were obtained during a bathing season from 47 seawater stations and from 1 estuarine station. To determine viral pollution, all samples were subjected to two different methods of viral concentration: tangential ultrafiltration and adsorption-elution with electropositive membranes. Detection of viruses was by cytopathic effect (CPE) in BGM and NA 104 cells. Isolates were identified by dot-blot hybridization and Polyacrylamide Gel Electrophoresis (PAGE). While estuarine water showed enterovirus and/or reovirus presence in 100% of samples, only 14 stations of 47 seawater station samples (30%) showed viral contamination: enteroviruses were isolated from 6 and reoviruses from 8 of the 14 stations. 28 unidentified viruses were detected from seawater in MA104 cells by CPE whereas these viruses were not detected in BGM cells. Enterovirus recovery seems to be better when water samples are concentrated by the tangential ultrafiltration than with absorption-elution with electropositive membranes. For reoviruses and the other viruses the two methods were almost equivalent. BGM cells seem to be more susceptible to enteroviruses, MA104 to reoviruses. Reoviruses failed to indicate enterovirus presence as most of enteroviruses were isolated in waters where reovirus was not observed. Isolated viral species distribution changed during bathing season.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Water, air & soil pollution 91 (1996), S. 327-334 
    ISSN: 1573-2932
    Keywords: enteric viruses ; domestic sewages ; enteroviruses ; reoviruses ; waste water treatment ; raw and treated sewages
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Over the course of a year a series of samples were taken from a wastewater treatment plant handling domestic sewage at each stages of the process, to detect the presence of enteric viral and classical bacterial indicators, and physicochemical parameters. The viruses were isolated on BGM cell cultures and counted according to the Most Probable Number method. The values of enteric viruses varied from 102 to 104/L in raw sewage and from 100 to 103 in final effluent. The efficiency of the plant at each stages during processing was evaluated. The parameters analysed show a systematic reduction of values between input and output, with average bacteriological reductions of 88% (fecal streptococci), 93% (fecal coliforms) and 94% (total coliforms), viral load reduced by 0–99%. COD and suspended solids showed a reduction of 61 % and 71% respectively. The 40% of isolated viruses were submitted to identification procedures using molecular techniques and pools of antisera. The viral types identified were enteroviruses (poliovirus and coxsackievirus B) and reoviruses. Viruses appear less easily removed than classical bacterial indicators. Reoviruses were removed less efficiently than enteroviruses.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This study is aimed to verify hygienic conditions of a commercially-important clam: Chamelea (Venus) gallina, widely distributed along the Italian coasts. Five mussel beds have been simultaneously surveyed, collecting samples of water, sediment and molluscs. Total coliforms, fecal coliforms, and fecal streptococci have been analyzed. Results show that this shellfish does not seem to accumulate enteric bacteria in spite of the microbial contamination of water and sediment.
    Type of Medium: Electronic Resource
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