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  • 1
    Electronic Resource
    Electronic Resource
    Erratum to: ‘Persistence of a Salmonella enterica serotype Typhimurium clone in Danish pig production units and farmhouse environment studied by pulsed field gel electrophoresis (PFGE)’ (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 189 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09252.x
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  • 2
    Electronic Resource
    Electronic Resource
    Persistence of a Salmonella enterica serotype Typhimurium clone in Danish pig production units and farmhouse environment studied by pulsed field gel electrophoresis (PFGE) (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 187 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The clonal relationship among Salmonella enterica serotype Typhimurium isolates from selected pig production units in Denmark was investigated by the pulsed field gel electrophoresis (PFGE) typing method to determine environmental survival and spread of Salmonella in different herds. Thirty-four Typhimurium isolated during 1996–1998 from porcine faeces and environmental samples from three pig farms designated 1, 3 and 5 were characterised by PFGE using two restriction enzymes. Farm 5 supplied piglets to farm 1 and the herds were located close to each other. Results of PFGE analysis showed both intra- and inter-relationships, i.e. identical PFGE patterns among the faecal and environmental isolates from farm 1 and farm 5. All the isolates from farm 3 irrespective of the source showed identical PFGE patterns, but were different from samples from farms 1 and 5. This study indicates spread between farms and survival of a farm-specific clone. Furthermore, identical PFGE patterns of isolates from piglet supplier and finisher herds indicate that the farrow-to-grower herd of farm 5 was sub-clinically infected prior to delivery to farm 1 and thereby caused the transmission of Salmonella.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09130.x
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  • 3
    Electronic Resource
    Electronic Resource
    Characterisation of potential virulence markers in Aeromonas caviae isolated from polluted and unpolluted aquatic environments in Morocco (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 27 (1998), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A total of 100 Aeromonas caviae strains isolated from river waters (38 isolates), raw sewage (30 isolates) and effluents of stabilisation ponds (i.e. treated sewage; 32 isolates) in Marrakech, Morocco, were tested for the presence of putative virulence factors to delineate differences, if any, in their enteropathogenic potential in relation to the source of isolation. A number of A. caviae isolates were able to elaborate at least one of the tested virulence factors. Of the 100 strains tested, 27 were capable of producing haemolysins, 19 produced cytotoxin, 24 produced cytotonic toxin, while 18 of the isolates possessed the capacity to adhere to HenLe 407 human intestinal cells with more than five bacteria adhering per cell. In order to assess the virulence potential of A. caviae isolates in relation to the source of isolation, we suggest the potential virulence index (PVI) as a tool for comparison. It is calculated as PVI=x/ny, where x is the number of positive occurrence of virulence factors in a population y, and n is the number of suitable virulence factors used for the calculation of the PVI. Accordingly, in this study A. caviae strains isolated from treated sewage samples showed a higher PVI (=0.406) than those isolated from raw sewage (PVI=0.175) or those from river waters (PVI=0.09). These results suggest that stabilisation pond systems used for sewage purification under arid climate conditions in Marrakech, may have selected potentially enteropathogenic A. caviae strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1998.tb00533.x
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  • 4
    Electronic Resource
    Electronic Resource
    Fibronectin binding and cell-surface hydrophobicity of attaching effacing enteropathogenic Escherichia coli strains isolated from newborn and weanling rabbits with diarrhoea (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 34 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract 32 different strains of Escherichia coli isolated from rabbits with diarrhoea were studied for cell-surface properties which may be involved in intestinal colonisation. Strains isolated from diarrhoeic suckling (6 strains) and weaning (26 strains) rabbits which were shown to attach to brush borders in vivo, showed high relative cell-surface hydrophobicity as determined by the Salt Aggregation Test (SAT) when grown on Colonisation Factor Antigen (CFA) agar at 33°C. Cells of these strains grown to express surface hydrophobicity were also defined as high, moderate or low binders of 125I-fibronectin or its 125I-29-kDa fragment in a standard binding assay. Based on these findings, we propose that binding to intestinal cell surface (mucus)-associated fibronectin may be an early important step in intestinal colonisation of the small bowel in enteropathogenic E. coli (EPEC) diarrhoea in rabbits and other animal species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01409.x
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  • 5
    Electronic Resource
    Electronic Resource
    Improvement of the salt aggregation test to study bacterial cell-surface hydrophobicity (1985)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 30 (1985), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract An improved salt aggregation test (improved SAT) was developed to sensitize the determination of bacterial cell-surface hydrophobicity. One drop of a fresh bacterial suspension standardized to an A1cm540 of 20 (equivalent to 5 × 109 cfu/ml), and one drop each of ammonium sulphate solutions stained with methylene blue, were mixed on a white hydrophobic paper card using toothpicks. The bacterial suspensions, methylene blue stock solutions and the ammonium sulphate solutions (0.01–4.0 M) were made in 0.02 M sodium phosphate buffer, pH 6.8. Bacterial aggregations were read immediately after mixing the salt/bacterial suspensions while the card was gently rocked. Readings were also confirmed the next day on dried preparations. The results proved independent of reading time and mixture conditions (wet or dry preparations). The improved SAT technique is very rapid and sensitive, the reaction is easily read with the naked eye, and the paper cards can be stored for documentation of aggregation patterns after drying. In the improved SAT, the Staphylococcus cells of different species aggregated in 5 ways: tiny, medial, flaky granular, particulated and macrofilamentous forms; Salmonella strains aggregated in flaky granular, particulated and macrofilamentous forms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1985.tb00999.x
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  • 6
    Electronic Resource
    Electronic Resource
    Fibronectin binding to Salmonella strains (1985)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 28 (1985), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The binding of 125I-labelled fibronectin and its 125I-labelled amino-terminal (29 kDa) domain to smooth Salmonella strains and rough variants was studied. 11 Of the 14 strains bound fibronectin, in the order of 20–70% of total radioactivity added. The kinetics of binding to Salmonella strain 27655R for the intact fibronectin and the 29 kDa fragment was very rapid, binding being completed within 20 min. Binding was specific, since the unlabelled fibronectin and the 29 kDa fragment could compete efficiently for binding of 125I-labelled fibronectin and fragment, whereas other glycoproteins, such as ovalbumin, fetuin and orosomucoid, showed very little or no inhibition. Since the unlabelled 29 kDa fragment could efficiently inhibit 125I-labelled fibronectin binding, it seems likely that the site of interaction is the amino-terminal domain of fibronectin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1985.tb00752.x
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  • 7
    Electronic Resource
    Electronic Resource
    Rapid detection of a new cell-associated Salmonella cytotonic enterotoxin in cell sonicates by Chinese hamster ovary cells (1983)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 20 (1983), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1983.tb00117.x
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  • 8
    Electronic Resource
    Electronic Resource
    Characterization of fibronectin binding to Salmonella enteritidis strain 27655R (1988)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 49 (1988), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The optimal conditions for the binding of fibronectin to Salmonella enteritidis strain 27655R, and the cell-surface components involved in the binding, were identified. Cultivation on colonisation factor antigen (CFA) agar or in CFA broth at 33°C for 24 h were found to be optimal for the expression of fibronectin binding. Such cultures exhibited 88% and 70% binding of 125I-labelled fibronectin and its 29-kDa N-terminal domain, respectively. The fibronectin binding was reversed by the addition of unlabelled fibronectin or its 29-kDa fragment. Scatchard plot analysis of the binding showed that the strain possessed one high-affinity (Kd= 5.8 × 10−10 M) and one low-affinity (Kd= 2 × 10−8 M) binding site. The fibronectin-binding could be inhibited by cell surface components of S. enteritidis 27655R released by 30 min treatment at 65°C or 95°C. Inhibition could also be achieved using purified fimbriae. A non-fimbriated mutant of strain 27655R showed a much reduced binding of fibronectin (15%). Electron microscopic analysis showed association of the gold-labelled 29-kDa N-terminal fragment with S. enteritidis 27655R fimbriae. In conclusion, the findings suggest that S. enteritidis (strain 27655R) possesses fibronectin-binding fimbriae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb02781.x
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