ISSN:
0006-3525
Keywords:
thermodynamic parameters of drug binding
;
1H-nmr of drug binding
;
ethidium bromide binding (ΔG, ΔH, ΔS)
;
intercalation of ethidium bromide
;
oligodeoxynucleotides
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The thermodynamical parameters (free energy, enthalpy, and entropy) of complex formation between ethidium bromide and single-stranded and double-stranded tetranucleotides of different base sequence [5′-d(TpGpCpA), 5′-d(ApCpGpT), and 5′-d(ApGpCpT) have been determined from the temperature dependencies of 500 MHz proton nmr chemical shifts. The analysis enables the contributions to be differentiated for the formation of different types of complexes (1:1, 2:1, 1:2, and 2:2) in aqueous solution. The results have been interpreted in terms of the main types of intermolecular interactions responsible for formation of the different complexes; van der Waals and electrostatic interactions are important for formation of complexes of ethidium bromide with single-stranded tetranucleotides, whereas van der Waals and hydrophobic interactions play a significant role in the binding of the dye to the tetramer duplexes. © 1997 John Wiley & Sons, Inc. Biopoly 42: 285-295, 1997
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
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