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  • 1
    Electronic Resource
    Electronic Resource
    Biological Sciences: Quinacrine Staining of Chromosomes and Evolutionary Studies in Drosophila (1971)
    [s.l.] : Nature Publishing Group
    Nature 233 (1971), S. 190-191 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] (1) Drosophila melanogaster differs consistently from its sibling species D. simulans in the pattern of the brightly fluorescent regions. The two species can be distinguished by the patterns of bright fluorescence of either the mitotic chromosomes of the neuroblasts or the polytene chromosomes of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/233190a0
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  • 2
    Electronic Resource
    Electronic Resource
    Differences in the quinacrine staining of the chromosomes of a pair of sibling species: Drosophila melanogaster and Drosophila simulans (1971)
    Springer
    Chromosoma 34 (1971), S. 424-435 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The patterns of intense fluorescence after staining with quinacrine dihydrochloride were studied in both the polytene chromosomes and mitotic chromosomes of a pair of sibling species, Drosophila melanogaster and D. simulans. Consistent differences between the two species were found in the pattern of fluorescence of both polytene and mitotic chromosomes. In addition, it was discovered that our stock of D. melanogaster (Oregon-R) is polymorphic at one autosomal position for the property of intense fluorescence after quinacrine staining. On the basis of these findings, the usefulness of quinacrine staining in the study of the cytogenetic structure of evolutionarily interesting populations is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326314
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  • 3
    Electronic Resource
    Electronic Resource
    Quinacrine fluorescence of specific chromosome regions (1972)
    Springer
    Chromosoma 36 (1972), S. 375-390 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The pattern of intense fluorescence of interphase nuclei and metaphase chromosomes after staining with quinacrine is described in Samoaia leonensis. Autoradiographic analysis of interphase nuclei after pulse labeling with tritiated thymidine indicates that there is little or no overlap in the time of replication of the intensely fluorescing and weakly fluorescing regions. Autoradiographic analysis of metaphase figures after continuous labeling with tritiated thymidine shows that the intensely fluorescing regions are late replicating and establishes their order of replication. Autoradiographic analysis of interphase nuclei after pulse labeling with tritiated deoxycytidine and of metaphase figures after continuous labeling with this tracer show that there is little, if any, incorporation of deoxycytidine into those chromosome regions which fluoresce intensely after staining with quinacrine and quinacrine mustard. These results indicate that such chromosome regions are characterized chemically by an extremely high, if not exclusive, content of adenine and thymine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336794
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  • 4
    Electronic Resource
    Electronic Resource
    The DNA content of sperm of Drosophila melanogaster (1971)
    Springer
    Chromosoma 33 (1971), S. 1-18 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Amounts of Feulgen staining in individual sperm nuclei of Drosophila melanogaster were determined with a scanning microdensitometer and compared with the DNA-Feulgen levels found for hen erythrocyte nuclei, taken as a presumed cytophotometric standard of 2.5×10−12 g DNA per cell. Under these conditions, the amount of DNA estimated for the haploid male genome of Drosophila was about 0.18×10−12 g DNA, corresponding to a molecular weight of roughly 11× 1010 daltons. From a comparable series of measurements on Feulgen-stained nuclei of hemocytes and epithelial cells of the imaginal soma, values of approximately 0.35–0.36×10−12 g DNA were estimated for the diploid or 2C male genome of this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326379
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  • 5
    Electronic Resource
    Electronic Resource
    Characterization of the toromeric structure in Drosophila lummei (1976)
    Springer
    Chromosoma 58 (1976), S. 63-71 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The toromere, a structure previously reported only in several strains of Drosophila melanogaster, is found in salivary gland nuclei of three populations of Drosophila lummei, a member of the virilis group. The toromere is characterized by being quinacrine-bright and Feulgen-positive. Further staining and enzyme digestion procedures reveal that the toromere is composed of double-stranded DNA with little or no protein complexed with it. The toromere appears as a small quinacrine-bright dot in diploid cells and apparently undergoes polytenization, as it is observed as a large quinacrine-bright doughnut-shaped structure in salivary gland preparations. We describe the effect of larval culture temperature on the morphology and number of toromeres per cell, and lend support to the proposal that the toromere could be a highly replicated episome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293441
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  • 6
    Electronic Resource
    Electronic Resource
    Ectopic pairing of chromosome regions containing chemically similar DNA (1972)
    Springer
    Chromosoma 39 (1972), S. 53-61 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Using genetically controlled stocks ofDrosophila melanogaster we have compared the frequency of ectopic pairing in a line showing intense quinacrine fluorescence at two sites (81F and 83E) on chromosome 3 with one showing such fluorescence at only one of these sites (81F). The frequency of ectopic pairing is an order of magnitude greater in cells from the line showing intense fluorescence in both regions than in the line showing it in only one. These data indicate that ectopic pairing is dependent upon properties of discrete chromosome regions as small as individual bands. Since A: T-rich chromatin is known to fluoresce intensely after quinacrine staining, these data further suggest that ectopic pairing is dependent on similarities of the DNA of the discrete chromosome regions involved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320590
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  • 7
    Electronic Resource
    Electronic Resource
    Comparative morphology of nucleolar DNA in Drosophila. II. Drosophila fulvimaculoides and drosophila tumiditarsus (1971)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 134 (1971), S. 243-257 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This paper reports the demonstration, using fluorescence microscopy, of nucleolar DNA in two species of Drosophila. In Drosophila fulvimaculoides, the nucleolar DNA presents a variable morphology, suggestive of puffing activity. This material, which sometimes shows a banded structure like that of the polytene chromosomes, is shown not to be coextensive with the Y chromosome. Nucleolar DNA is demonstrated in Drosophila tumiditarsus also, and previous reports of an association of the dot chromosome with the nucleolus in this species are confirmed. The special usefulness of these two species for various sorts of investigation in pointed out.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051340207
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  • 8
    Electronic Resource
    Electronic Resource
    An effect of exogenous thymidine on the mitotic cycleThis paper is dedicated to the memory of Professor L. V. Heilbrunn, in whose laboratory at Woods Hole the author first became interested in the problems of cell division. (1963)
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 61 (1963), S. 119-127 
    Details
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1030610203
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