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  • 1
    Electronic Resource
    Electronic Resource
    Assembly Properties of Neurofilament Light Chain Ser55 Mutants in Transfected Mammalian Cells (1996)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 66 (1996), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Ser55 within the head domain of neurofilament light chain (NF-L) is transiently phosphorylated by protein kinase A, and phosphorylation of this residue is thought to regulate assembly of neurofilaments. To understand how Ser55 phosphorylation influences NF-L assembly, wild-type and mutant NF-L genes in which Ser55 was mutated to alanine, so as to prevent phosphorylation, or to aspartate, so as to mimic permanent phosphorylation, were transfected into mammalian cells that contain or do not contain an endogenous intermediate filament network. Wild-type and mutant NF-Ls localised to the Triton X-100-insoluble fraction, which suggests that phosphorylation of Ser55 does not inhibit assembly of NF-L and NF-L/vimentin polymers at or below the tetrameric stage. Immunofluorescence microscopy of transfected cells demonstrated that the wild-type and mutant NF-Ls all colocalised with vimentin to produce similar filamentous arrays. However, in cells lacking an endogenous intermediate filament network, the aspartate mutant produced a pattern of staining different from that of the wild-type or alanine mutant. These results suggest that phosphorylation of NF-L Ser55 is not a mechanism that precludes assembly of neurofilaments from monomers into intermediate filament structures but that phosphorylation/dephosphorylation of this residue might confer more subtle characteristics on neurofilament assembly properties and architecture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1996.66031306.x
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  • 2
    Electronic Resource
    Electronic Resource
    Neuropathological Abnormalities in Transgenic Mice Harbouring a Phosphorylation Mutant Neurofilament Transgene (1998)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 70 (1998), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Ser55 within the head domain of neurofilament light chain (NF-L) is a target for phosphorylation by protein kinase A. To understand further the physiological role(s) of NF-L Ser55 phosphorylation, we generated transgenic mice with a mutant NF-L transgene in which Ser55 was mutated to Asp so as to mimic permanent phosphorylation. Two lines of NF-L(Asp) mice were created and these animals express the transgene in many neurones of the central and peripheral nervous systems. Both transgenic lines display identical, early onset, and robust pathological changes in the brain. These involve the formation of NF-L(Asp)-containing perikaryal neurofilament inclusion bodies and the development of swollen Purkinje cell axons. Development of these pathologies was rapid and fully established in mice as young as 4 weeks of age. The two transgenic lines show no elevation of NF-L, neurofilament middle chain (NF-M), or neurofilament heavy chain (NF-H), and transgenic NF-L(Asp) represents only a minor proportion of total NF-L protein. Because other published transgenic lines expressing higher levels of wild-type NF-L do not exhibit phenotypic changes that in any way resemble those in the NF-L(Asp) mice and because the two different NF-L(Asp) transgenic lines display identical neuropathological changes, it is likely that the pathological alterations observed in the NF-L(Asp) mice are the result of properties of the mutant NF-L. These results support the notion that phosphorylation of Ser55 is a mechanism for regulating neurofilament organisation in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1998.70020492.x
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  • 3
    Electronic Resource
    Electronic Resource
    Conversion of Glucose to Galactose in the Mammary Gland (1952)
    [s.l.] : Nature Publishing Group
    Nature 169 (1952), S. 878-879 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] 1-14C glucose containing 11-3 (xC. of carbon-14 was dissolved in saline and infused intravenously over 1 hr. into a lactating goat. The goat was milked just before the start of the infusion, and again 2J and 4J hr. later, and lactose was isolated from the last two milk samples. The lactose was ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/169878a0
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  • 4
    Electronic Resource
    Electronic Resource
    Chromatin dispersal and DNA synthesis in G1 and G2 HeLa cell nuclei injected into Xenopus eggs (1977)
    [s.l.] : Nature Publishing Group
    Nature 265 (1977), S. 469-470 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Figure 1 shows the size distribution of G1 and G2 HeLa cell nuclei 5, 60 and 120 min after injection of ruptured HeLa cells into Xenopus eggs. At 5 min, before the nuclei had swollen appreciably, they were all below 17-mm diameter. Also, the distribution of G1 and G2 nuclei between the two size ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/265469a0
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  • 5
    Electronic Resource
    Electronic Resource
    Biosynthesis of Gramicidin- S (1958)
    [s.l.] : Nature Publishing Group
    Nature 181 (1958), S. 1274-1274 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] When a culture of Bacillits brevis (A.T.C.C. No. 9999) was aerated at 37 C. in a medium supplemented with the five amino-acids of gramicidin-/ , a normal bacterial growth-curve was obtained. Biological assay with Streptococcus sp. (A.T.C.C. No. 9854) of alcoholic extracts of the bacteria showed ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1811274a0
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  • 6
    Electronic Resource
    Electronic Resource
    Use of Glutamine by the Mammary Gland for the Synthesis of Casein (1954)
    [s.l.] : Nature Publishing Group
    Nature 174 (1954), S. 315-316 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] 1-14C DL-glutamine was injected intravenously into a lactating goat and specific activities were measured in various components of blood and milk taken at intervals up to 12 hr. after injection. During the first few hours the specific activity of the free glutamine of plasma remained many times ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/174315b0
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  • 7
    Electronic Resource
    Electronic Resource
    Species Specificity of Fish Mucus (1959)
    [s.l.] : Nature Publishing Group
    Nature 184 (1959), S. 2039-2039 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The chromatographic patterns of flatfish hybrids were intermediate between those of the parents but tended more towards the maternal type. Vis-wanathan and Krishna Pillai3, using tissue crushes from fishes, dead for some time, demonstrated clear differences between pairs of species in the genera ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1842039a0
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  • 8
    Electronic Resource
    Electronic Resource
    Cadaver kidney transplantation in patients more than 65 years old (1996)
    Springer
    World journal of urology 14 (1996), S. 243-248 
    Details
    ISSN: 1433-8726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Elderly patients with end-stage renal disease often remain on dialytic therapy because they are at increased risk for mortality and morbidity. We placed 24 cadaver kidney transplants into 24 patients aged 65–74 years between September 1, 1985, and August 31, 1995. Rates of patient and graft survival were compared with those of 404 concurrent first cadaver-kidney transplant recipients between the ages of 20 and 44 years. The 5-year rates of patient and graft survival were not significantly different (86% versus 92% and 77% versus 63%, respectively; study group presented first). Primary cadaver kidney transplantation can be successfully performed in patients older than 65 years when a selection algorithm is applied to exclude active infection, active malignancy, unsuitable anatomy for technical success, high probability of operative mortality, and noncompliance. Pelvic arteriosclerosis and lower urinary tract abnormalities can cause intraoperative technical problems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00182075
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  • 9
    Electronic Resource
    Electronic Resource
    Asparagine in Blood Plasma (1953)
    [s.l.] : Nature Publishing Group
    Nature 171 (1953), S. 1123-1123 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] If an amino-acid mixture is analysed by chromato-graphy on 'Dowex-50' resin exactly as described by Moore and Stein3, but at 20 C. instead of 37-5 C, glutamine and asparagine emerge as a single peak. When a blood plasma dialysate is heated to 100 C. at pH 6-5 for two hours so as to remove glutamine ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1711123a0
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  • 10
    Electronic Resource
    Electronic Resource
    Use of IS, TWA and ILWS (1973)
    [s.l.] : Nature Publishing Group
    Nature 242 (1973), S. 284-284 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR,-The present author (PA) is writ-ing to request the increased use in Nature of initial-letter^word-substitu-tion (ILWS). Unpublished studies of the PA show that the superficial-scienti-fic-appearance-rating (SSAR) of a paper is most effectively increased by im-personal style (IS), ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/242284a0
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