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  • 1
    Electronic Resource
    Electronic Resource
    An analysis of genetic diversity in coconut (Cocos nucifera) populations from across the geographic range using sequence-tagged microsatellites (SSRs) and AFLPs (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 764-771 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetic diversity in 31 individuals from 14 coconut populations across the entire geographic range (2–3 individuals per population) was assessed using sequence-tagged microsatellites (or simple sequence repeats, SSRs) and amplified fragment length polymorphism (AFLP). From the 39 SSR primer sets tested, only two gave patterns that could not be scored and used in the data analysis. The remainder included five SSRs that gave double-locus profiles in which one locus could still be scored separately. The 37 SSRs revealed between 2 and 16 alleles per locus and a total of 339 alleles in the 14 populations. Gene diversity (D = 1-Σpi 2) ranged from 0.47 to 0.90. Two of the four Dwarf populations were homozygous at all 37 loci, which is consistent with their autogamous (self-fertilising) reproduction. One Dwarf population was heterozygous at one locus but the other (Niu Leka Dwarf), which is known to be cross-pollinating, showed high levels of heterozygosity. Generally, diversity was higher in populations from the South Pacific and South East Asia. Three SSR loci (CNZ46, CN2A5, CN11E6) gave distinct genotypes for all but two populations. The East African populations had higher heterozygosities than those from West Africa, and the populations from Tonga and Fiji generally had distinct alleles from those of the South Pacific. AFLP analysis with 12 primer combinations gave a total of 1106 bands, of which 303 were polymorphic (27%). Similarity matrices were constructed from the two data sets using the proportion of shared alleles for SSRs and a Jaccard coefficient for AFLPs. In each case cluster and principal co-ordinates analyses were performed, with the resultant dendrograms and plots revealing similar relationships among the populations for both approaches. There was generally a good separation of populations, and phenetic relationships were in agreement with those previously shown by RFLPs. The use of SSRs and AFLPs in genetic-diversity analysis for the establishment of germplasm collections is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051350
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  • 2
    Electronic Resource
    Electronic Resource
    A new aspect of genetic diversity of Indonesian oil palm (Elaeis guineensis Jacq.) revealed by isoenzyme and AFLP markers and its consequences for breeding (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 956-961 
    Details
    ISSN: 1432-2242
    Keywords: Keywords Elaeis guineensis ; AFLP ; Isoenzyme ; RRS ; Genetic structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Oil palm (Elaeis guineensis Jacq.) plays an important economic role in some countries of Southeast Asia like Indonesia, which is the world’s second producer of palm and palm kernel oil. The quality improvement of planting material needs a better understanding of the genetic relationships between genotypes from different populations used in the breeding programmes. In this study, 48 parents, representative of four populations used in Indonesian Oil Palm Research Institute (IOPRI) breeding programmes, were analysed with five selected AFLP primer pairs and four isoenzymatic systems. One hundred and fifty eight scorable band levels were generated of which 96 (61%) were polymorphic. AFLP allowed us to identify off–type descendants which were excluded from analysis. The use of unbiased Rogers distance clearly separated the four studied populations. The Neighbor-Joining method re-groups two African populations which are known as originating from different regions. Nevertheless, the variability revealed is in accordance with oil palm breeders’ knowledge. The results obtained with AFLP showed that the crosses among the African sub-population, which is excluded in oil palm reciprocal recurrent selection (RRS) breeding programmes, may be more interesting than the crosses between the African and the Deli populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051567
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of the genetic effects for several traits in oil palm (Elaeis guineensis Jacq.) populations. I. Population means (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 561-570 
    Details
    ISSN: 1432-2242
    Keywords: Population structure ; Genetic components of the mean ; Crossing plans ; Reciprocal recurrent selection ; Elaeis guineensis Jacquin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An oil palm experiment was set up in the Ivory Coast to compare the effects of crossing and selfing within two origins, Deli and La Mé, on the mean and the variability of Deli x Mé between-origin hybrids. The originality of the experiment lay in the crossing plan, which provided access to genetic parameters related to additivity, dominance and different components of epistasis. This first part covers the analysis of the components of the mean. The parents used were obtained from four palms, two from each origin. Those of La Mé origin were half-sibs. The common parent came from a wild stand in the Ivory Coast. Those of Deli origin were from two different populations bred in Southeast Asia for several generations from a narrow genetic base. These four parents gave rise to nine Deli x La Mé hybrid populations with double-cousintype links. The additive component is more important within the Deli origin than within the La Mé origin. This may be explained by the large genetic divergence between the two Deli parent palms. On the other hand, the additive* additive epistasis is more substantial within the La Mé origin, probably because of inbreeding. The discussion concentrates on how this information should be used when choosing parents to be crossed and tested and to produce improved populations. The crossing plan proposed can be of general use and is suitable for other species in a reciprocal recurrent selection programme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222004
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic diversity in coconut (Cocos nucifera L.) revealed by restriction fragment length polymorphism (RFLP) markers (1998)
    Springer
    Euphytica 101 (1998), S. 103-108 
    Details
    ISSN: 1573-5060
    Keywords: Cocos nucifera ; RFLP ; genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A study of the genetic diversity in coconut by RFLP analysis was performed in 100 individuals representing 10 Tall and seven Dwarf local populations or 'ecotypes' from various geographical origins. Nine cDNA clones from rice, one mitochondrial DNA clone (CoxI) and one genomic clone (rDNA) from wheat were used as probe for southern hybridization. The distribution of the 40 polymorphic bands revealed by rice cDNA clones was studied using a multivariate analysis and allowed to identify two main genetical groups. The first one includes the ecotypes from the Far East and from the South Pacific, whereas the other one comprises the ecotypes from India, Sri Lanka and Western Africa. The rDNA and the CoxI probes confirm this distinction. The Far East and the Pacific regions which were the most likely center of origin also exhibit the widest polymorphism. The associations between the Panama Tall and the Pacific group and between the West African Tall and the Indian Ocean group reflect their likely origin. The Comoro Tall appears to be intermediate between the two main groups, and could reflect the old migration route between Indonesia and Madagascar. All Dwarf varieties belong to the first group, even those collected in West Africa. Those were probably introduced from Asia and Pacific at the beginning of the century. The cross-pollinating Tall ecotypes were generally more polymorphic than the self pollinating Dwarf ecotypes. The legitimacy of two hybrids between ecotypes was confirmed and maternal inheritance of mitochondrial genome was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018323721803
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    Paper (German National Licenses)
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