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  • 1
    Electronic Resource
    Electronic Resource
    Genetically Engineered Protection Against Viruses in Transgenic Plants (1993)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 47 (1993), S. 739-763 
    Details
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.mi.47.100193.003515
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Coat Protein-Mediated Resistance Against Virus Infection (1990)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Phytopathology 28 (1990), S. 451-472 
    Details
    ISSN: 0066-4286
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.py.28.090190.002315
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Optimization of parameters for particle bombardment of embryogenic suspension cultures of cassava (Manihot esculenta Crantz) using computer image analysis (1997)
    Springer
    Plant cell reports 16 (1997), S. 526-530 
    Details
    ISSN: 1432-203X
    Keywords: Key words Embryogenic suspension cultures ; β-Glucuronidase ; Microparticle bombardment ; Image analysis ; Ferricyanide ; ferrocyanide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tissue derived from embryogenic suspension cultures of cassava was bombarded with microparticles coated with a plasmid containing the uidA gene, which codes for β-glucuronidase (GUS). After 3 days, the effect of different bombardment parameters was evaluated by comparing the numbers of blue spots that resulted from histological GUS assays. Counting of blue spots was performed using a system comprised of a black and white video camera, a stereoscope and a personal computer. A reproducible counting method was established by optimizing GUS assay conditions, preparation of tissue samples and acquisition of video images in view of attaining the highest possible contrast between the blue spots and the surrounding tissue. The effects of bombardment pressure, microparticle size, number of bombardments, and osmotic pretreatment on GUS expression were investigated. Optimal transient expression of the uidA gene was observed after bombardment at 1100 psi, with a particle size of 1 µm, an osmotic pretreatment and two bombardments per sample. The highest number of blue spots observed was 2400 per square centimeter of bombarded tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01142317
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Regeneration of transgenic cassava plants (Manihot esculenta Crantz) through Agrobacterium-mediated transformation of embryogenic suspension cultures (1998)
    Springer
    Plant cell reports 17 (1998), S. 827-831 
    Details
    ISSN: 1432-203X
    Keywords: Key words Embryogenic suspension cultures ; β-Glucuronidase ; Agrobacterium tumefaciens ; Cassava ; Paromomycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A protocol was developed for Agrobacterium-mediated transformation of embryogenic suspension cultures of cassava. The bacterial strain ABI containing the binary vector pMON977 with the nptII gene as selectable marker and an intron-interrupted uidA gene (encoding β-glucuronidase) as visible marker was used for the experiments. Selection of transformed tissue with paromomycin resulted in the establishment of antibiotic-resistant, β-glucuronidase-expressing lines of friable embryogenic callus, from which embryos and subsequently plants were regenerated. Southern blot analysis demonstrated stable integration of the uidA gene into the cassava genome in five lines of transformed embryogenic suspension cultures and in two plant lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050492
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  • 5
    Electronic Resource
    Electronic Resource
    A protocol for consistent, large-scale production of fertile transgenic rice plants (1998)
    Springer
    Plant cell reports 18 (1998), S. 25-31 
    Details
    ISSN: 1432-203X
    Keywords: Key words Rice ; Microparticle bombardment ; Genetic transformation ; Embryogenic tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A protocol for consistent production of fertile transgenic rice plants was established utilizing microparticle bombardment of embryogenic tissues (Oryza sativa L. japonica cv. Taipei 309). This system has been employed to produce several thousand independently transformed plant lines carrying the hygromycin phosphotransferase (hph) gene and various genes of interest. The most efficient target tissue was highly embryogenic callus or suspension cell aggregates, when they were given an osmotic pre- and post-transformation treatment of 0.6 m carbohydrate. By optimizing the age of the tissue at the time of gene transfer and applying an improved selection procedure, transgenic plants were recovered in 8 weeks from the time of gene transfer, at an average of 22.3±9.7 per 100 calli and 22.4±8.0 plant lines per dish of suspension cell aggregates. This system has facilitated a number of studies using rice as a model for genetic transformation and will enable the large-scale production of transgenic rice plants for genomic studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050526
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  • 6
    Electronic Resource
    Electronic Resource
    An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants and axillary bud-derived meristems (1997)
    Springer
    Plant cell reports 16 (1997), S. 444-449 
    Details
    ISSN: 1432-203X
    Keywords: Micropropagation ; Pluronic F-68 ; Multiple shoots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nodes from 3- to 5-week-old in vitro plants of different cassava cultivars were cultured for 2–3 days on solid Murashige and Skoog basal medium supplemented with cytokinin to induce the enlargement of axillary buds. Subculture of these buds on the same medium resulted in multiple shoot formation within 4–6 weeks. Of the four cytokinins tested (6-benzylaminopurine (BAP), thidiazuron (TDZ), zeatin, and kinetin), BAP induced shoot development most efficiently. The best results were obtained with cultivar TMS 30555, in which 63% of the explants each produced at least 25 shoots on medium with 10 mg/l BAP. In cultivars that did not produce shoots, the addition of the surfactant Pluronic F-68 (2% wt/vol) raised the percentage of explants forming at least 5 shoots from 0 to 20–60%. Axillary buds were also used to dissect meristems and test their ability to regenerate into shoots. Shoot formation from meristems of six different cultivars was observed after preculture on medium with 5 mg/l BAP followed by transfer to 10 mg/l BAP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092763
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  • 7
    Electronic Resource
    Electronic Resource
    Expression of storage-protein genes during soybean seed development (1981)
    Springer
    Planta 153 (1981), S. 130-139 
    Details
    ISSN: 1432-2048
    Keywords: Embryonic axis ; Glycine ; mRNA accumulation ; Seed development ; Storage proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mature seeds of Glycine max (L.) Merr. contain two major storage proteins, a glycosylated 7S protein (conglycinin) and a non-glycosylated 11S protein (glycinin). Accumulation of these proteins and their mRNAs during seed development in cv. Provar was studied by SDS polyacrylamide gel electrophoresis and by “Northern” (DNA-RNA) hybridization. The 11S acidic and basic subunits and the 7S α′ and α subunits began to accumulate 18–20 d after pollination, shortly after the termination of cell division in developing cotyledons, whereas the 7S β and 11S A-4 subunits were not detected until one to two weeks later, during the maturation phase of development. Messenger RNAs for 7S and 11S proteins were first detected 14–18 d after pollination, several days before the accumulation of storage proteins. Extracts from embryonic axes contained reduced levels of the 7S α subunit, very little 11S protein, no detectable 7S β or 11S A-4 subunits, and an additional 7S subunit not found in cotyledons. Soybean axes and cotyledons therefore differ in their synthesis of seed storage proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384094
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Phosphodiesterase activities in transgenic tobacco plants associated with the movement protein of tobacco mosaic virus (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 730-734 
    Details
    ISSN: 1432-2242
    Keywords: Movement protein ; Phosphodiesterase ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hydrolytic activities of leaf extracts from normal and transgenic plants, with (+ MP) and without (-MP) the movement protein of tobacco mosaic virus, were examined. In the + MP transgenic plants, as compared with non-transgenic and — MP plants, higher hydrolytic activities were found on the following substrates: bis-(nitrophenyl)-phosphate (BPNPP, phosphodiesterase), p-nitrophenyl-(phenyl)-phosphate (PNPPP, nucleotidephosphodiesterase) and thymidine-3′-monophosphate p-nitrophenyl ester (T3MPP; 3′nucleotide phosphodiesterase.) The + MP plant lines, as compared with other transgenic plants, exhibited higher nucleotide-phosphodiesterase activity in the soluble as well as in the membrane fraction. Substrate concentration kinetic studies revealed the presence of a nucleotide-phospho-diesterase with a high substrate affinity in the +MP extracts in addition to the enzyme with a relatively low substrate affinity present also in the — MP transgenic plants. This “high affinity” enzyme could be removed from the soluble fraction by precipitation with anti-MP serum, indicating its possible association with the movement protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224177
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Optimization of parameters for particle bombardment of embryogenic suspension cultures of cassava (Manihot esculenta Crantz) using computer image analysis (1997)
    Springer
    Plant cell reports 16 (1997), S. 526-530 
    Details
    ISSN: 1432-203X
    Keywords: Embryogenic suspension cultures ; β-Glucuronidase ; Microparticle bombardment ; Image analysis ; Ferricyanide ; ferrocyanide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tissue derived from embryogenic suspension cultures of cassava was bombarded with microparticles coated with a plasmid containing theuidA gene, which codes forβ-glucuronidase (GUS). After 3 days, the effect of different bombardment parameters was evaluated by comparing the numbers of blue spots that resulted from histological GUS assays. Counting of blue spots was performed using a system comprised of a black and white video camera, a stereoscope and a personal computer. A reproducible counting method was established by optimizing GUS assay conditions, preparation of tissue samples and acquisition of video images in view of attaining the highest possible contrast between the blue spots and the surrounding tissue. The effects of bombardment pressure, microparticle size, number of bombardments, and osmotic pretreatment on GUS expression were investigated. Optimal transient expression of theuidA gene was observed after bombardment at 1100 psi, with a particle size of 1 µm, an osmotic pretreatment and two bombardments per sample. The highest number of blue spots observed was 2400 per square centimeter of bombarded tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01142317
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Improvement of somatic embryogenesis and plant recovery in cassava (1993)
    Springer
    Plant cell reports 12 (1993), S. 328-333 
    Details
    ISSN: 1432-203X
    Keywords: Manihot esculenta ; somatic embryogenesis ; maturation ; germination ; desiccation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methods for improving the efficiency of plant recovery from somatic embryos of cassava (Manihot esculenta Crantz) were investigated by optimizing the maturation regime and incorporating a desiccation stage prior to inducing germination. Somatic embryos were induced from young leaf lobes of in vitro grown shoots of cassava on Murashige and Skoog medium with 2,4-dichlorophenoxy acetic acid. After 15 to 20 days of culture on induction medium, the somatic embryos were transferred to a hormone free medium supplemented with activated charcoal. In another 18 days mature somatic embryos became distinctly bipolar and easily separable as individual units and were cultured on half MS medium for further development. Subsequent desiccation of bipolar somatic embryos resulted in 92% germination and 83% complete plant regeneration. The plants were characterized by synchronized development of shoot and root axes. Of the non-desiccated somatic embryos, only 10% germinated and 2% regenerated plants. Starting from leaf lobes, transplantable plantlets were derived from primary somatic embryos within 70 to 80 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237429
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