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  • 1
    Electronic Resource
    Electronic Resource
    Estimation of the number of full sibling families at an oviposition site using RAPD-PCR markers: applications to the mosquito Aedes aegypti (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 991-1000 
    Details
    ISSN: 1432-2242
    Keywords: RAPD-PCR ; DNA Fingerprinting ; Sibling analysis ; Oviposition ; Aedes aegypti
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There are many species in which groups of individuals encountered in the field are known to consist of mixtures of full-sibling families. We describe a statistical technique, based on the use of random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers, that allows for the estimation of the number of families contained in these groups. We test the technique on full-sibling families of the mosquito Aedes aegypti, a species that distributes its eggs among several locations. Mixtures of 10 families with 15 individuals per family were analyzed using 40 RAPD-PCR loci amplified by 5 primers. Our analysis accurately estimated the number of families. The technique was accurate when the number of families was small or when family sizes were small and variable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00211052
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  • 2
    Electronic Resource
    Electronic Resource
    Genomic stability of La Crosse virus during vertical and horizontal transmission (1989)
    Springer
    Archives of virology 108 (1989), S. 89-99 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have used ribonuclease T 1 oligonucleotide fingerprint analysis to study genomic stability of La Crosse virus (Bunyaviridae) during vertical and horizontal transmission in the laboratory. No RNA genomic changes were detected in vertebrate cell culture-propagated virus isolated (following ingestion and replication) from the natural host,Aedes triseriatus. Genomic changes were not detected during transovarial passage of the virus through two generations of mosquitoes, nor were changes detected in the genomes of virus isolated from suckling mice that had been fed upon by second generation transovarially-infected mosquitoes. These results demonstrate that despite the well-documented phenomena of rapid nucleotide change in RNA virus genomes under various conditions, the La Crosse virus genome can remain stable during transovarial transmission in the insect host and during transfer between the insect and vertebrate hosts. The evolutionary implications of these results are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01313746
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Use of the Sindbis replicon system for expression of LaCrosse virus envelope proteins in mosquito cells (1998)
    Springer
    Archives of virology 143 (1998), S. 1365-1377 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  The Sindbis replicon expression system was used to express La Crosse (LAC) virus envelope glycoprotein genes in both mammalian and mosquito cell culture. Replicon expressed LAC proteins had correct molecular mass (Mr) and were antigenically similar to wild type LAC envelope proteins. In addition, LAC G1 and G2 proteins colocalized when expressed from separate constructs in both mammalian and mosquito cells suggesting that they were trafficked through the cell similarly to wild type LAC proteins. A truncated form of the G1 protein was secreted from mosquito cells when expressed alone. The truncated G1 protein was also secreted from mosquito cells when expressed with the G2 protein, but to a lesser extent than when expressed alone, suggesting that the G2 protein sequestered G1 protein intracellularly. The Sindbis replicon system is a powerful tool for the study of LAC virus protein maturation within mosquito cells and mosquitoes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050381
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    Paper (German National Licenses)
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