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  • 1
    Electronic Resource
    Electronic Resource
    Quantitative separation of Trichoderma lipid classes on a bonded phase column
    Amsterdam : Elsevier
    Journal of Chromatography B: Biomedical Sciences and Applications 584 (1992), S. 129-133 
    Details
    ISSN: 0378-4347
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0378-4347(92)80018-L
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  • 2
    Electronic Resource
    Electronic Resource
    Fatty acid cellular metabolism and lactone production by the yeast Pichia guilliermondii (1996)
    Springer
    Applied microbiology and biotechnology 45 (1996), S. 349-354 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Methyl ricinoleate conversion into γ-decalactone by fungi is already widely used by the aromatic industry. It offers an interesting alternative to chemical synthesis by permitting acquisition of a natural label. Peroxisomal β-oxidation has been described as the probable transformation mechanism. This paper provides information about this metabolism and shows the importance of the step catalysed by carnitine octanoyltransferase. After culture of the yeast Pichia guilliermondii on a medium containing methyl ricinoleate as sole carbon source, we confirmed that mitochondrial β-oxidation could not be responsible for the biotransformation. We also observed the effect of chlorpromazine, an inhibitor of carnitine octanoyltransferase, on peroxisomal β-oxidation and therefore on lactone production, and on lipid accumulation by the yeasts. The presence of chlorpromazine caused a reduction in aromatic specific production yield. This reduction was inversely proportional to the amount of chlorpromazine present in the medium. A considerable accumulation of methyl ricinoleate derivatives was also observed. We therefore concluded that the metabolism responsible for the bioconversion was peroxisomal β-oxidation. The effects of chlorpromazine suggested that the entry of fatty acids into the peroxisomes took place in a carnitine-dependent manner. This step might be a limiting step in the metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050695
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  • 3
    Electronic Resource
    Electronic Resource
    Peroxisomal β-oxidation activities and γ-decalactone production by the yeast Yarrowia lipolytica (1998)
    Springer
    Applied microbiology and biotechnology 49 (1998), S. 295-300 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract γ-Decalactone is a peachy aroma compound resulting from the peroxisomal β-oxidation of ricinoleic acid by yeasts. The expression levels of acyl-CoA oxidase (gene deletion) and 3-ketoacyl-CoA thiolase activities (gene amplification on replicative plasmids) were modified in the yeast Yarrowia lipolytica. The effects of these modifications on β-oxidation were measured. Overexpression of thiolase activity did not have any effect on the overall β-oxidation activity. The disruption of one of the acyl-CoA oxidase genes resulted in an enhanced activity. The enhancement led to an increase of overall β-oxidation activity but reduced the γ-decalactone production rates. This seemed to indicate a non-rate-limiting role for β-oxidation in the biotransformation of ricinoleic acid to γ-decalactone by the yeast Yarrowia lipolytica. All strains produced and then consumed γ-decalactone. We checked the ability of the different strains to consume γ-decalactone in a medium containing the lactone as sole carbon source. The consumption of the strain overexpressing acyl-CoA oxidase activity was higher than that of the wild-type strain. We␣concluded that peroxisomal β-oxidation is certainly involved in γ-decalactone catabolism by the yeast Y.␣lipolytica. The observed production rates probably depend on an equilibrium between production and consumption of the lactone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051172
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  • 4
    Electronic Resource
    Electronic Resource
    Contribution a l'étude des levures des chais- taxonomie, répartition des levures (1979)
    Springer
    Mycopathologia 67 (1979), S. 67-81 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les populations levuriennes de deux chais de Bourgogne sont étudiées sur deux années d'un point de vue taxonomique et biologique. 122 souches se rapportant à 26 espèces, ont été isolées à partir de la voûte et du sol de la cave, sur les madriers supportant les tonneaux, dans la cuve, sur le pressoir et sur les tonneaux. En dehors d'un certain nombre d'espèces classiques dans ce type d'habitat, telles que Saccharomyces cerevisiae, Pichia membranaefaciens, Hansenula anomala et Kloeckera apiculata, nous avons pu mettre en évidence notamment: Debaryomyces hansenii, Torulopsis Candida, Saccharomyces italicus, ainsi qu'un certain nombre d'espèces appartenant aux genres Pichia, Candida et Trichosporon. La répartition qualitative et quantitative des espèces est également étudiée.
    Notes: Abstract The yeast populations of two burgundy cellars were studied during two years from a taxonomical and biological point of view. 122 strains distributed among 26 species have been isolated from the vault and ground of the cellar, on the planks supporting the barrels, in the tank, on the winepress and on the barrels. Apart from a number of standard species in this type of habitat such Saccharomy cescerevisiae, Pichia membranaefaciens, Hansenula anomala, Kloeckera apiculata we have encountered the more rare Debaryomyces hansenii, Torulopsis Candida, Saccharomyces italicus as also species of the genus Pichia, Candida, and Trichosporon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00440676
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  • 5
    Electronic Resource
    Electronic Resource
    Interet de la microscopie electronique a balayage pour l'etude morphologique des spores de levures (1971)
    Springer
    Mycopathologia 45 (1971), S. 253-257 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The morphology of spores is an important aspect of yeasts systematic. It is a good thing to state the size, the shape and the ornamentation of these spores; only scanning electron microscopy is able to bring a satisfactory answer. This preliminary work applied toEndomycopsis javanensis (Klöcker)Dekker spores shows that their warty aspect is due to the presence of warts and mamelons and that the width of the equatorial edge varies considerably for a given spore and from one spore to another.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02051972
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  • 6
    Electronic Resource
    Electronic Resource
    A study of the budding ofSaccharomyces uvarum Beijerinck with the scanning electron microscope (1972)
    Springer
    Antonie van Leeuwenhoek 38 (1972), S. 341-349 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Vegetative cells ofSaccharomyces uvarum Beijerinck in the exponential growth phase were examined with the scanning electron microscope. The existence of two types of scars — birth scars and bud scars — was confirmed. Birth scars had larger diameters than bud scars; both remained visible on old cells. The distribution of the buds on the mother cell did not appear to be a random one: there seemed to be a more or less emphasized cell polarity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02328103
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  • 7
    Electronic Resource
    Electronic Resource
    Effects of polyphenolic compounds on the growth and cellulolytic activity of a strain of trichoderma viride (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 983-989 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260240420
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  • 8
    Electronic Resource
    Electronic Resource
    Apple pomace: An enzyme system for producing aroma compounds from polyunsaturated fatty acids (1991)
    Springer
    Biotechnology letters 13 (1991), S. 893-898 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary This paper reports on the possibility of obtaining C-6 (hexanal) and C-10 (2,4-dedadienal) volatile aldehydes by degradation of linoleic acid (C18∶2 Δ 9–12) under the action of the intrinsic enzyme systems found in apple pomace. More aroma compounds are produced by micronization of the pomace and by adding SO2 (60 ppm) and vitamin C (500 ppm), thereby synergistically counteracting oxidation of phenolic compounds, which is a limiting factor in bioconversion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01022094
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  • 9
    Electronic Resource
    Electronic Resource
    Production of 6-pentyl-α-pyrone byTrichoderma harzianum from 18∶n fatty acid methyl esters (1992)
    Springer
    Biotechnology letters 14 (1992), S. 1019-1024 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Biosynthesis of 6-pentyl-α-pyrone byTrichoderma harzianum in two different media was evaluated. Best yields were found in nitrogen deficient medium (C/N=60). Limited growth seems to favour the production of this lactone. When fungal cells, precultured in low nitrogen medium, were incubated on methyl ricinoleate (10 g/l, C/N=60) an increase in 6-pentyl-α-pyrone production was observed in comparison with the media containing methyl oleate or methyl linoleate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01021051
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