ISSN:
0192-253X
Keywords:
sperm
;
F9 antigen
;
T/t-complex
;
immunolabeling
;
scanning electron microscopy
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The antigens defined by conventional syngeneic antiserum against F9 embryonal carcinoma cells were localized on mature sperm using immunolabeling and scanning electron microscopy. Labeling patterns were compared for normal (+ / +) mice and mice bearing recessive t-haplotypes. The results showed that antigens detected by intact anti-F9 antiserum are expressed similarly in all genotypes, except for sperm from mice bearing the t12-haplotype where the frequency of labeled cells was reduced. Labeling with the IgM fraction of anti-F9 antiserum was lower on sperm from all t-genotypes examined, with sperm from + /t12 males showing the most marked reduction. In all cases, the labeling patterns were similar, and included a labeling of the whole sperm head with complete anti-F9 antiserum and a restriction of the label to the postacrosomal region when the IgM fraction was used.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/dvg.1020020204
Permalink