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  • 1
    Electronic Resource
    Electronic Resource
    Clomiphene citrate and hMG: An alternative stimulation protocol for selected failed in vitro fertilization patients (1995)
    Springer
    Journal of assisted reproduction and genetics 12 (1995), S. 8-12 
    Details
    ISSN: 1573-7330
    Keywords: in vitro fertilization ; clomiphene citrate ; ovarian stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose This study was carried out to evaluate the potential role of the combination clomiphene citrate/human menopausal gonadotropin (CC/hMG) for patients who failed previous in vitrofertilization (IVF) attempts with gonadotropin-releasing hormone analogs (GnRH- a) and/ or exogenous gonadotropins. Methods Patients were stimulated with CC/hMG (n=93) after unsuccessfully undergoing 182 gonadotropin cycles with (n=106) or without (n=76) luteal-phase GnRH- a. Cancellation rate, length of stimulation, and peak estradiol levels did not differ significantly between the two regimens. Results Although fewer oocytes were retrieved when the CC/hMG combination was used, 16 patients were able to successfully achieve a pregnancy (26.2% delivery rate/ transfer). When daily follicle stimulating hormone (FSH) levels were measured in two successive cycles in those women who conceived with the CC/hMG stimulation, baseline levels did not differ when compared with a previous GnRH-a/hMG cycle. Nevertheless, serum FSH levels rose rapidly and remained higher in the GnRH- a/hMG cycle, reaching significantly higher levels on day of human chorionic gonadotropin (hCG) administration. Conclusion Selected patients who failed previous IVF attempts with gonadotropins with or without GnRH analogs may benefit from the addition of CC to their ovarian stimulation protocol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02214121
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Bovine serum albumin (BSA) can replace patient serum as a protein source in an in vitro fertilization (IVF) program (1989)
    Springer
    Journal of assisted reproduction and genetics 6 (1989), S. 164-167 
    Details
    ISSN: 1573-7330
    Keywords: bovine serum albumin (BSA) ; human in vitro fertilization (IVF)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Alternate protein sources have been suggested to replace the commonly used cord or patient serum for in vitro fertilization (IVF) procedures. During an 11-month period 127 patients treated for in vitro fertilization had elther their serum (N= 71) or bovine serum albumin (BSA;N= 56) used as the protein source in the insemination and growth media. Ham's F-10+0.5% BSA was used for sperm swim-up and insemination media and 1% BSA was used for the growth media. Patient's serum was added to Ham's F-10 culture media at concentrations of 7.5 and 15% for insemination and growth, respectively. Embryo transfer was performed with Ham's F-10 containing 90% maternal serum in both groups. Fertilization rate of 259 oocytes inseminated in medium containing patient's serum did not differ when compared with 200 oocytes inseminated in medium containing BSA. Likewise, rates of obnormal fertilization, cleavage, and pregnancy were similar in both groups. In a second experiment, 148 normally fertilized oocytes were transferred after 24 hr in culture to growth media containing two different concentrations of BSA (0.5 or 1%). Cleavage rates for the two groups were similar and the percentage of embryas developed to ≧4 cells did not differ signficantly. We conclude that a single concentration of BSA can safely be used to supplement culture media in human IVF with several practical and economical benefits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01130782
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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