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  • 1
    Digitale Medien
    Digitale Medien
    Comparative NMR studies of diffusional water permeability of erythrocytes from some animals introduced to Australia: Rat rabbit and sheep (1994)
    Springer
    Comparative clinical pathology 4 (1994), S. 232-235 
    Details
    ISSN: 1433-2981
    Schlagwort(e): NMR ; Rabbit ; Rat ; Red blood cells ; Sheep ; Water permeability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The diffusional water permeability (P d) of the red blood cell (RBC) membrane of rat, rabbit and sheep, representing some animal species introduced to Australia, has been monitored, by a Mn2+-doping 1H nuclear magnetic resonance (NR) technique using a high-field spectometer operating at 400 MHz. In order to make comparisons with previous measurements on the same species (performed at 25 MHz) an analysis of the influence of instrumental parameters and of the state of blood oxygenation was performed on samples of rat and rabbit blood. It was found that by using a short interpulse delay (around 100 µs) in the Carr-Purcell-Meiboom-Gill sequence, and by performing the measurements of the transverse relaxation time of the water in the cell interior (T 2i) on packed cells (haematocrit 〉95%), the values of the water exchange time (T e) obtained with a high-field spectrometer are comparable with those obtained by using a low-field NMR spectrometer. The values of the diffusional water permeability (P d), calculated from the T e values, were, at 24.6°C around 10 × 10−3 cm/s in case of rat and rabbit RBC and around 5 × 10−3 cm/s for sheep RBC; at 37°C the P d values were around 16 × 10−3 cm/s for rat RBC, 14 × 10−3 cm/s for rabbit RBC and 7 × 10−3 cm/s for sheep RBC. These values are very close to the P d values previously reported for the European counterparts of these species. There were no significant differences in the P d values between laboratory rabbits and wild rabbits, or between castrated males, pregnant or nonpregnant females. These results suggest that no changes in the RBC water permeability are correlated with marked alterations in the habitat of animal species introduced to Australia or by sex hormone status. If the NMR instrument-parameters are carefully chosen then comparative studies of RBC water permeability in different laboratories, at separate locations and using different instruments are meaningful.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00185179
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  • 2
    Digitale Medien
    Digitale Medien
    Comparative Nuclear Magnetic Resonance Studies of Diffusional Water Permeability of Red Blood Cells from Different Species. XI. Horses Introduced to Australia and European Horses (Equus caballus) (2000)
    Springer
    Comparative clinical pathology 10 (2000), S. 138-143 
    Details
    ISSN: 1433-2981
    Schlagwort(e): Key words:Aquaporin – Horse – NMR – Red blood cells – Water permeability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract: The diffusional water permeability (P d) of red blood cells (RBCs) from horses introduced to Australia and from European horses was measured by a Mn2+ doping nuclear magnetic resonance (NMR) technique. The values of P d were ∼3.5 × 10−3 cm/s at 25°C, 4.1 × 10−3 cm/s at 30°C, 5.6 × 10−3 cm/s at 37°C and 6.3 × 10−3 cm/s at 42°C with no significant differences between the two strains of horse. Systematic studies on the effect of p-chloromercuribenzene sulphonate (PCMBS) on water diffusion indicated that the maximal inhibition (60%) was reached in 60 min at 22°C with 1 mm PCMBS. The basal permeability to water was estimated at 1.2 × 10−3 cm/s at 25°C, 1.7 × 10−3 cm/s at 30°C, 2.0 × 10−3 cm/s at 37°C and 2.7 × 10−3 cm/s at 42°C. The activation energy (E a,d) of water diffusion was ∼25 kJ/mol and increased to ∼39 kJ/mol after incubation with PCMBS, in conditions of maximal inhibition of water diffusion. The membrane protein electrophoretic pattern of horse RBCs was compared with its human counterpart. The horse RBC membranes had lower amounts of the proteins migrating as bands 4.1 and 4.2 and higher amounts of the protein migrating as band 4.9, and band 6 (glyceraldehyde-3-phosphate dehydrogenase) was undetectable. A noteworthy feature was the appearance of considerable differences in protein migration distances in the region of bands 7 and 9, between horse and human membranes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s005800070005
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  • 3
    Digitale Medien
    Digitale Medien
    Comparative NMR Studies of Diffusional Water Permeability of Red Blood Cells from Different Species. X. Camel (Camelus dromedarius) and Alpaca (Lama pacos) (1999)
    Springer
    Comparative clinical pathology 9 (1999), S. 43-48 
    Details
    ISSN: 1433-2981
    Schlagwort(e): Key words:Alpaca – Aquaporin – Camel – NMR – Red blood cells – Water permeability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract: The diffusional water permeability (P d) of camel and alpaca red blood cells (RBCs) was measured by a doping nuclear magnetic resonance (NMR) technique on control cells and following inhibition with p-chloromercuribenzene sulphonate (PCMBS). The values of P d were, in the case of alpaca RBC ≈ 4.6 × 10−3 cm/s at 25°C, 5.4 × 10−3 cm/s at 30°C, 6.6 × 10−3 cm/s at 37°C and 7.7 × 10−3 cm/s at 42°C. In case of camel RBC the values of P d where ≈ 4.2 × 10−3 cm/s at 25°C, 5.3 × 10−3 cm/s at 30°C, 7.4 × 10−3 cm/s at 37°C and 9.0 × 10−3 cm/s at 42°C. Systematic studies on the effects of PCMBS on water diffusion in camel RBC indicated that the maximal inhibition was reached in 45 min with 1–2 mm PCMBS. The values of maximal inhibition were around 47% at 25°C and 68% at 30°C for alpaca RBC and around 62% at 25°C and 56% at 37°C for camel RBC. The basal permeability to water of alpaca RBC was estimated at around 2.6 × 10−3 cm/s at 25°C, 1.7 × 10−3 cm/s at 30°C and of camel RBC as 1.8 × 10−3 cm/s at 25°C and 3.0 × 10−3 cm/s at 37°C. The values of the activation energy of water diffusion (E a,d) were around 23 kJ/mol for camel and 34 kJ/mol for alpaca RBC. This suggests that in addition to the number of transport channels other features of the pathways might be important for defining the temperature dependence of the water permeability.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/PL00010006
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  • 4
    Digitale Medien
    Digitale Medien
    Scanning electron microscopy of red blood cells from eleven species of marsupial (1992)
    Springer
    Comparative clinical pathology 2 (1992), S. 227-230 
    Details
    ISSN: 1433-2981
    Schlagwort(e): Erythrocytes ; Marsupials ; Red blood cells ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Samples of red of blood cells (RBC), washed free of plasma, from eleven marsupial species were examined in a Jeol JSM-6300 F scanning electron microscope. The diameters of the RBC, lying completely flat or exactly on edge, were measured on photographs using a binocular enlarging optical system with a calibrated eye piece. RBC from the following species were studied: bandicoot (Isoodon macrourus), bilby (Macrotis lagotis sagitta), Bennett's wallaby (Macropus rufogriseus), Goodfellow's tree kangaroo (Dendrolagus goodfellowi), koala (Phascolarctos cinereus), parma wallaby (Macropus parma), red kangaroo (Macropus rufus), swamp wallaby (Wallabia bicolor), Tammar wallaby (Macropus eugenii), Tasmanian devil (Sarcophilus harrish) and whiptail wallaby (Macropus parryi). In all species the RBC were biconcave discs. The major morphological difference was in the size of the cells. Taking the human RBC as a reference (with a diameter of 8.0 μm), the RBC of the Tasmanian devil, bilby, bandicoot and Goodfellow's tree kangaroo were ∼7 μm in diameter, whereas those of the other marsupials ranged from 7.8 to 8.6 μm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216099
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