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  • 1
    Electronic Resource
    Electronic Resource
    Transport des Hydroxyanalogons von Leucin in Bürstensaum-Membranvesikel des Kaninchendünndarms (1991)
    Springer
    European journal of nutrition 30 (1991), S. 233-237 
    Details
    ISSN: 1436-6215
    Keywords: Hydroxyanaloga von Aminosäuren ; H+-Ionen-stimulierter Transport ; Bürstensaum-Membranvesikel ; hydroxy analogues of amino acids ; proton driven transport ; brushborder membran vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary Hydroxy analogues of essential amino acids can be used in clinical nutrition to minimize nitrogen intake. In this study intestinal uptake of L-leucine hydroxy analogue into rabbit jejunal brush-border membrane vesicles was investigated. An inward directed H+-gradient was a driving force of uptake (pHoutside = 6.0; pHinside = 7.5) and led to a transient accumulation. The saturable system has a apparent transport constant Kt = 15.4 mM. By trans stimulation experiments it could be shown that both D- and L-stereoisomers of hydroxy analogues of branched chain amino acids as well as L-lactate share with the same H+-driven uptake system.
    Notes: Zusammenfassung Hydroxyanaloga essentieller Aminosäuren können ebenso wie ihre Ketoanaloga zur Minimierung der nutritiven Stickstoffaufnahme verwandt werden. In der vorliegenden Arbeit werden Mechanismen der intestinalen Absorption des L-Hydroxyanalogons von Leucin untersucht. Die Aufnahme dieses Substrates in Bürstensaum-Membranvesikel des jejunalen Kaninchendünndarms wird durch einen nach innen gerichteten Protonengradienten stimuliert (pHaußen 6,0; pHinnen 7,5). Die scheinbare Transportkonstante beträgt 15,4mM. Durch das carriervermittelte Transportsystem werden gleichfalls L- und D-Stereoisomere der anderen Hydroxyanaloga verzweigtkettiger Aminosäuren sowie L-Lactat aufgenommen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01610347
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  • 2
    Electronic Resource
    Electronic Resource
    Affinity chromatography of soluble galactosyltransferases using an easily synthesized N-acetylglucosamine-agarose bead (1976)
    Springer
    Cellular and molecular life sciences 32 (1976), S. 690-691 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Agarose derivatized withp-nitrophenyl-N-acetyl-β-d-glucosamine was used for affinity chromatography for soluble galactosyltransferase from various sources. Moreover, this adsorbant acts as an acceptor for galactose when incubated with prepurified galactosyltransferase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01919834
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  • 3
    Electronic Resource
    Electronic Resource
    Golgi-disturbing agents (1998)
    Springer
    Histochemistry and cell biology 109 (1998), S. 571-590 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Pharmacological agents have proven useful for gaining fundamental insights into the biology of the Golgi apparatus. This review summarizes pertinent and recent work on the effects on this organelle of monensin, brefeldin A, bafilomycin, ilimaquinone, okadaic acid, retinoic acid, and nocodazole. The molecular targets of monensin, brefeldin A, ilimaquinone, and retinoic acid remain to be elucidated whereas those for bafilomycin (vacuolar H+-ATPase), okadaic acid (serine/threonine phosphatases types 1, 2a, and 2b), and nocodazole (microtubules) are reasonably well understood. The molecular target of brefeldin has not been defined, but has been suggested to involve guanine nucleotide exchange proteins acting on ADP-ribosylation factor 1. Whether a defined molecular target can be found for monensin must be questioned since its main action consists in exchanging protons for Na+ which leads to osmotic swelling of post-Golgi endosomal structures and Golgi subcompartments by virtue of its membrane-associated effect as a cationophore. Brefeldin A was one of the most thoroughly investigated Golgi-disturbing agents and proved instrumental in unraveling retrograde flow mechanisms in the secretory pathways. Okadaic acid attracted interest for its properties mimicking mitotic fragmentation of the Golgi apparatus. Nocodazole was instrumental in establishing the cytoskeletal anchoring of the Golgi apparatus close to the microtubular organizing center.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050256
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  • 4
    Electronic Resource
    Electronic Resource
    Immunohistochemical localization of α1-acid-glycoprotein in human liver parenchymal cells (1977)
    Springer
    Histochemistry and cell biology 51 (1977), S. 293-296 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary α1-acid glycoprotein, a major human serum glycoprotein was detected and localized in human liver parenchymal cells of a biopsy specimen. A heavy metal salt containing fixative was required to retain sufficient antigen determinants of α1-acid glycoprotein in order to visualize this protein by the peroxidase-anti-peroxidase unlabeled antibody enzyme method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494364
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  • 5
    Electronic Resource
    Electronic Resource
    Die Wirkung oral verabreichten Butyl-Biguanids auf die orale Glucosetoleranz und renale Glucoseresorption (TmG) beim gesunden Menschen in vivo (1975)
    Springer
    Journal of molecular medicine 53 (1975), S. 275-278 
    Details
    ISSN: 1432-1440
    Keywords: Biguanides ; glucose tolerance test ; kidney tubulus ; kidney function test ; Biguanide ; Glucosetoleranztest ; Nierentubuli ; Nierenfunktionsprüfung ; Glucosetransportsysteme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 1. Die orale Glucosetoleranz wird nach peroraler Verabreichung von 150 mg Butyl-Biguanid bei 11 Normalpersonen signifikant verbessert. 2. Demgegenüber ändern sich das Transportmaximum der renalen Glucoseresorption (TmG) und der Schwellenwert der renalen Glucosefiltration, bei welchem eine Glucosurie auftritt (Fmin) bei 7 Normalpersonen nicht. 3. Diese Resultate sind vereinbar mit der Hypothese, daß die perorale Gabe der Biguanide in einer therapeutischen Dosis zu einer Hemmung der intestinalen Glucoseresorption infolge der hohen Konzentrationen im Darmsaft führt. Die Serumkonzentrationen der oral zugeführten Biguanide genügen für eine in vivo nachweisbare Hemmung der renalen Glucoseresorption nicht. 4. Diurese und Inulinclearance bleiben bei Normalpersonen durch peroral zugeführtes Butyl-Biguanid unbeeinflußt.
    Notes: Summary 1. Oral glucose tolerance in 11 normal subjects was significantly improved after peroral administration of 150 mg of butyl-biguanide. 2. On the other hand maximum capacity of renal glucose reabsorption (TmG) and lowest glomerular glucose filtration rate at which glucosuria was recorded (Fmin) remained uninfluenced after 150 mg of butyl-biguanide in 7 normal subjects. 3. These results are compatible with the hypothesis that the oral administration of therapeutic doses of biguanides yields concentrations at the luminal face of the intestine which inhibit glucose resorption. Plasma concentrations following oral intake of the same dose of biguanide are too low to exert any inhibiting action on renal glucose reabsorption as demonstrated by the present in vivo studies. 4. Urinary flow rate and inulin clearance remained unaltered after butyl-biguanide in these normal subjects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01469119
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  • 6
    Electronic Resource
    Electronic Resource
    S13.10 Generation of normal and glycosylphosphatidyl inositol (GPI)-deficient T-lymphocyte clones from a patient with paroxysmal nocturnal hemoglobinuria (1993)
    Springer
    Glycoconjugate journal 10 (1993), S. 307-307 
    Details
    ISSN: 1573-4986
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01210072
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  • 7
    Electronic Resource
    Electronic Resource
    S14.4 Molecular organization of thetrans-golgi apparatus: Evidence for different compartents of β1,4-galactosyl- and α-2,6-sialyltransferase in HEPG2 cells (1993)
    Springer
    Glycoconjugate journal 10 (1993), S. 311-311 
    Details
    ISSN: 1573-4986
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01210085
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  • 8
    Electronic Resource
    Electronic Resource
    Purification of bovine colostrumβ-galactosideα(2–6)sialyltransferase to near homogeneity by affinity chromatography (1984)
    Springer
    Glycoconjugate journal 1 (1984), S. 141-153 
    Details
    ISSN: 1573-4986
    Keywords: sialyltransferase ; colostrum ; CDP-ethanolamine-(aminocaproyl)-agarose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Cytidine-5′-monophospho-N-acetylneuraminic acid:β-galactoside α2-6sialyltransferase was purified from bovine colostrum by two sequential affinity chromatography steps on CDP-ethanolamine-Sepharose and CDP-ethanolamine-(N-caproylamino-)-Sepharose, respectively. While the conditions for elution were those of Paulsonet al. [J Biol Chem (1977) 252:3256–62], the ligand of the second affinity column was coupled to Sepharose by using 6-aminocaproic acid as linker. The ease of this procedure allows rapid synthesis of bulk quantities of ligand. Highly purified preparations of sialyltransferase were obtained which moved on gradient gel electrophoresis as a single band of 76 kDa and on dodecylsulphate electrophoresis as a single band of 54 kDa. The product of the reaction between lactose and CMP-N-acetylneuraminic acid catalyzed by the purified sialyltransferase was identified by high-resolution 500 MHz1H-NMR spectroscopy as Neu5Acα2-6Galβ1-4Glc.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01213727
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  • 9
    Electronic Resource
    Electronic Resource
    S6.5 Heterologous expression of membrane-bound and soluble human β,4 galactosyltransferase and α2,6 sialyltransferase inSaccharomyces cerevisine (1993)
    Springer
    Glycoconjugate journal 10 (1993), S. 258-259 
    Details
    ISSN: 1573-4986
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01209922
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  • 10
    Electronic Resource
    Electronic Resource
    Investigation of the acceleration and running-down of a rigid rotor, rotating in hinged and elastic supports (1977)
    Springer
    International applied mechanics 13 (1977), S. 271-275 
    Details
    ISSN: 1573-8582
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00882679
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