ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Glycerol 3-phosphate cytidylyltransferase from Staphylococcus aureus (TarDSa) has been expressed in Escherichia coli, purified to homogeneity and crystallized. The strategy used for determining crystallization conditions employed hanging-drop sparse-matrix screens and required a combination of three different optimization approaches. Specifically, the presence or absence of cofactors needed to be surveyed, the effects of small-molecule additives required exploration and the rate of vapour-diffusion had to be varied in order to obtain crystals of TarDSa suitable for diffraction studies. Crystals thus obtained belong to the space group P3121, with unit-cell parameters a = b = 92.2, c = 156.1 Å, and contain four TarDSa molecules per asymmetric unit. The resolution limit observed for these crystals using synchrotron radiation is 3.0 Å.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444901005212
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