ISSN:
1432-203X
Keywords:
Brassica oleracea
;
rapid cycling cabbage
;
transformation
;
Agrobacterium rhizogenes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Genetically transformed cabbage (Brassica oleracea var. capitata) roots were obtained after inoculation with two engineered Agrobacterium rhizogenes strains, each harbouring a plant selectable marker gene in their T-DNA. Axenic root clones resistant to kanamycin or hygromycin B were established, most of which did not exhibit the phenotypic characteristics of Ri-transformed roots. Shoot regeneration was induced from roots after treatment with 2,4-dichlorophenoxyacetic acid (2,4-D). The resulting plants exhibited various phenotypes: some looked normal, while others showed the transformed phenotype observed in other species. Direct evidence for genetic transformation was obtained by molecular hybridization. The trait was transmitted to the progeny. Transformed cabbage plants can be obtained within 6 months using this approach.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00233360
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