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  • 1
    Electronic Resource
    Electronic Resource
    Ecdysone synthesis and release by the brain-ring gland complex of blowfly larvae
    Amsterdam : Elsevier
    Journal of Insect Physiology 34 (1988), S. 563-569 
    Details
    ISSN: 0022-1910
    Keywords: Ecdysone ; GABA ; azadirachtin ; hormone synthesis ; neurotransmitter ; opioid-like peptide ; ring gland
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0022-1910(88)90060-1
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  • 2
    Electronic Resource
    Electronic Resource
    Progestagen nuclear binding sites in the male genital tract of the mouse, studied by autoradiography
    Amsterdam : Elsevier
    The @Journal of Steroid Biochemistry and Molecular Biology 46 (1993), S. 389-393 
    Details
    ISSN: 0960-0760
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0960-0760(93)90229-P
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  • 3
    Electronic Resource
    Electronic Resource
    Vitamin D (Soltriol) receptors in the choroid plexus and ependyma: Their species-specific presence
    Amsterdam : Elsevier
    Molecular and Cellular Neuroscience 2 (1991), S. 145-156 
    Details
    ISSN: 1044-7431
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/1044-7431(91)90007-B
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  • 4
    Electronic Resource
    Electronic Resource
    Ecdysteroid receptors in the neuroendocrine-endocrine axis of a moth (1992)
    Springer
    Cellular and molecular life sciences 48 (1992), S. 42-47 
    Details
    ISSN: 1420-9071
    Keywords: Ecdysteroid receptor ; prothoracicotropic hormone ; neuroendocrine-endocrine axis ; feedback regulation ; autoradiography ; immunocytochemistry ; Manduca sexta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract With a combination of thaw-mount autoradiography using a tritiated 20-hydroxyecdysone agonist, ponasterone A, and immunocytochemistry with a monoclonal antibody to 29 K-prothoracicotropic hormone, high affinity binding sites for ecdysteroids were identified in the tissues of the neuroendocrine-endocrine axis inManduca sexta larvae. At specific times during larval-pupal development in fifth stadium larvae, nuclear ecdysteroid binding sites were present in the cerebral prothoracicotropes, the corpora allata and prothoracic glands, the main axis for the regulation and production of ecdysteroids. A stage-specific appearance of ecdysteroid receptors also occurred in cells of fat body, midgut and Malpighian tubules, tissues which convert ecdysone into 20-hydroxyecdysone. Our data identify new target tissues for ecdysteroids and suggest that ecdysteroids could affect their own production at the genomic level via long and short feedback loops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01923604
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  • 5
    Electronic Resource
    Electronic Resource
    Ecdysteroid receptors located in the central nervous system of an insect (1989)
    Springer
    Cellular and molecular life sciences 45 (1989), S. 106-109 
    Details
    ISSN: 1420-9071
    Keywords: Ecdysteroid ; steroid hormone receptor ; central nervous system ; interendocrine regulation ; autoradiography ; fly
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using thaw-mount autoradiography for steroid hormones, we obtained direct evidence for a nuclear localization of ecdysteroid binding sites in target organs of blowfly (Calliphora vicina) larvae. The binding sites revealed properties of ecdysteroid receptors. Endocrine cells of the ring gland were found to be target tissues of ecydysteroids. This observation provides morphological evidence for a network of complex interendocrine regulation. In the central nervous system receptorcontaining neurons were identified which include many, if not all, neurosecretory cells of the brain. A map of ecdysteroid sensitive cells of the larval brain is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01990464
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  • 6
    Electronic Resource
    Electronic Resource
    Retinoic acid binding sites in adult brain, pituitary, and retina (1991)
    Springer
    Naturwissenschaften 78 (1991), S. 561-562 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01134449
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  • 7
    Electronic Resource
    Electronic Resource
    Differential maturational patterns of nitric oxide synthase-I and NADPH diaphorase in functionally distinct cortical areas of the mouse cerebral cortex (1999)
    Springer
    Anatomy and embryology 200 (1999), S. 27-41 
    Details
    ISSN: 1432-0568
    Keywords: Key words Brain ; Cortical parcellation ; Development ; Proteoglycans ; Apoptosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Nitric oxide (NO) regulates several functions both in the developing and the adult central nervous systems (CNS). During development, NO is assumed to contribute to the histogenetic differentiation of the CNS especially through the modulation of programmed neuronal death. The embryonal and postnatal changes in the distribution of the cortical NO producing system were studied in Balb/c mice using immunocytochemistry for nitric oxide synthase-I (NOS-I) and NADPH-diaphorase (NADPH-d) enzyme histochemistry. NOS-I reactive neurons (RN) appeared first at embryonic day 14 (E14) in the spinal cord in the vicinity of the central canal, and later, at E16–18, in the thalamus and striatum. The first cortical region to present NOS-I reactivity was the parietal cortex, which happened at E18–20. After E20 the number of NOS-I RN increased in every cortical area, plateauing at postnatal day 4 (P4). In parietal regions, however, the highest density of NOS-I RN was observed already at P1. The neuronal packing density (PD) of NOS-I RN declined until adulthood, interrupted by a transient increase in some cortical areas at the onset of puberty. The heterochronous appearance of NOS-I during pre- and postnatal development of different brain regions and the sequence of up- and downregulation of expression until adult stages points to an important role of NO in brain development and functional differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004290050256
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  • 8
    Electronic Resource
    Electronic Resource
    Vitamin D receptors in heart: Effects on atrial natiuretic factor (1991)
    Springer
    Cellular and molecular life sciences 47 (1991), S. 958-962 
    Details
    ISSN: 1420-9071
    Keywords: Vitamin D receptor ; heart ; atrial natriuretic factor ; mouse ; autoradiography ; soltriol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We report that receptors for vitamin D exist in distinct regions of the heart in female and male mice, predominantly in the right atrium where most of the cardial atrial natriuretic peptide (ANF) is produced. Tritiated 1,25-dihydroxyvitamin D3 (1,25-D3, vitamin D, soltriol) and ANF are colocalized in nuclei and cytoplasm respectively in identical cardiomyocytes. Changes of ANF tissue and blood levels under dietary deficiency and treatment with 1,25-D3 suggest direct genomic actions of vitamin D on myoendocrine cells of the atrium for the regulation of ANF manufacture and secretion. These results were obtained by combining thaw-mount autoradiography with immunocytochemistry using tritiated 1,25-D3 and an antibody against rat ANF. This antibody was also used in a radioimmunoassay to determine atrial natriuretic factor in plasma, atria and ventricles of normal or vitamin D-deficient mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01929891
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  • 9
    Electronic Resource
    Electronic Resource
    Colocalization of atrial natriuretic factor (ANF) and estradiol in hypothalamic neurons by combined autoradiography-immunohistochemistry (1990)
    Springer
    Histochemistry and cell biology 94 (1990), S. 505-508 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of estrogen target neurons which contain atrial natriuretic factor (ANF) in female rat hypothalamus was investigated by thaw-mount autoradiography combined with immunocytochemistry using tritium-labeled estradiol and antibodies against ANF. Colocalization of the two hormones was found in the arcuate nucleus, periventricular nucleus, lateral ventromedial nucleus, ventral premammillar nucleus and lateral basal hypothalamus. The percentage of ANF containing cells which concentrate estradiol varies among the different hypothalamic nuclei with the highest number of ANF-positive cells showing nuclear concentration of 3H-estradiol (80–90%) in the nucleus premammillaris ventralis, but less (5–15%) in the other nuclei. These data, together with topographical correspondence in extrahypothalamic brain regions between sites of action of estradiol and production of ANF, suggest extensive interrelationships and modulatory effects of estradiol on ANF production and secretion in the brain, similar to the atrium of the heart.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272614
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  • 10
    Electronic Resource
    Electronic Resource
    Ecdysteroid binding sites in gastrolith forming tissue and stomach during the molting cycle of crayfishProcambarus clarkii (1992)
    Springer
    Histochemistry and cell biology 98 (1992), S. 1-6 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of ecdysteroid binding sites in the stomach and gastrolith disc tissue of cryafish (Procambarus clarkii) was examined in relation to the molting stage by thaw-mount autoradiography. The radiolabeled hormone analogue ponasterone A (25-deoxy-20-hydroxyecdysone) was used. Ecdysteroid binding sites were demonstrated only in certain molting stages, the small gastrolith period and the aftermolt stage. In gastrolith epithelium, ponasterone A binding sites first appeared in the cytoplasm, and then in the nuclei and cytoplasm. In the stomach epithelium, many nuclear binding sites were detectable during the period of gastrolith secretion. These periodical changes in specific ponasterone A binding when correlated with the molting stages clearly show that ecdysteroids may function as an initiator for gastrolith formation and reabsorption. The findings also suggest that ecdysteroids control calcium transport in the stomach epithelium. The time-related and functional differences of cytoplasmic and nuclear concentration of ecdysteroid receptors indicate the presence of cytoplasmic and nuclear receptors associated with specific actions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716931
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