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  • 1
    Electronic Resource
    Electronic Resource
    Machine vision based analysis and harvest of somatic embryos
    Amsterdam : Elsevier
    Computers and Electronics in Agriculture 9 (1993), S. 13-23 
    Details
    ISSN: 0168-1699
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Computer Science , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0168-1699(93)90026-W
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Automated, in vitro harvest of somatic embryos (1994)
    Springer
    Plant cell, tissue and organ culture 39 (1994), S. 171-183 
    Details
    ISSN: 1573-5044
    Keywords: classification ; machine vision ; somatic embryogenesis ; sorting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This work has demonstrated the aseptic, automated harvest of somatic embryos from a bioreactor suspension culture. Machine vision, emulating the selection criteria of an experienced biologist, classified embryos as harvestable or non-harvestable as they flowed through a 3 mm glass conduit. Embryos classified as harvestable were separated in a sealed harvest chamber. The system harvested 60% of the embryos at a rate of 2.4 embryos/h and incorrectly harvested less than 1% of the non-harvest objects. The low harvest rate precludes the applicability of this technique to research and commercial tissue culture laboratories. The suspension feed-rate, culture population density and culture homogeneity were identified as the most important factors influencing embryo harvest rate. The performance of this technique on more densely populated cultures was projected using anticipated improvements in suspension feedrate. It was concluded that, under the conditions of this analysis, the harvester would be of limited value in a commercial propagation environment but could be beneficial to many research labs working with plant somatic embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033924
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Noninvasive evaluation of somatic embryogenesisFlorida Agricultural Experiment Station, Journal Series No. R-01924. (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 39 (1992), S. 378-383 
    Details
    ISSN: 0006-3592
    Keywords: callus suspension cultures ; machine vision system ; Ipomea batatas ; somatic embryogenesis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Callus Suspension Cultures of Ipomea batates Poir. cv. White Star were grown in an airlift bioreactor. A machine vision system was used to monitor nondestructively callus growth during a 10 day culture period. Growth data obtained with this system included the overall reactor population and population estimates for the 200-1200-μm fractions at 200-μm intervals. A model of callus growth was developed to explain the mechanics of callus enlargement. The model was based on the assumptions that (1) the calli could not subdivide or shrink, (2) there was a fixed percentage of the initial population for each fraction that was nonviable, and (3) growth rates did not vary with time during the culture period. It was determined that the growth rates and nonviable ratios decreased as fraction size increased.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260390403
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    Paper (German National Licenses)
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