ISSN:
1439-0523
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Protoplasts of eight wild Linum species and 14 genotypes of flax, isolated from different parts of seedlings and from shoot apices, were regenerated to callus in medium V-KM (Binding and Nehls 1977). Plant regeneration was obtained in L. alpinum, L. amurense, L. hologynum, L. perenne, L. salsoloides and eight genotypes of L. usitatissimum on agar media B5 (Gamborg et al. 1968), B5C (Binding et al, 1981), MS (Murashige and Skoog 1962) and MSC (MS with 5% coconut endosperm) containing 2.5μM 6-BA. An adventitious embryo was regenerated in L. alpinum. Callus differentiation like sponge parenchyma, was observed in protoplast-derived tissues of L. usitatissimum. Shoots organized roots under slightly divergent conditions in the different species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1439-0523.1987.tb01135.x
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