ISSN:
1435-4373
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract U-373MG astrocytoma cells are susceptible to human cytomegalovirus (CMV) infection and offer the advantage of a continuous cell line for clinical laboratory use. U-373MG to MRC-5 cells for detection of CMV by centrifugation culture were therefore compared. At 20 h, 10 (6.1 %) versus 12 (7.4 %) of 163 clinical specimens were positive for CMV, and at 40 h, 12 (7.4 %) versus 17 (10.4 %) were positive in U-373MG and MRC-5 cells, respectively. Substantial toxicity was found in U-373MG cells (84 %) when inoculated with blood specimens. For detection of CMV in centrifugation culture, MRC-5 cells are superior due both to higher sensitivity and lesser toxicity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01967276
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