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  • 1
    ISSN: 1438-3888
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Distribution of hydrocarbons from the “Amoco Cadiz” oil spill in sublittoral sediments, north of Brittany. The quantitative distribution of hydrocarbons in the sublittoral sediments, north of Brittany (France), was followed and recorded for the first year after the spill (March, 1978). Following preliminary observations in spring of 1978, 250 stations were sampled in August from the Bay of Lannion to Portsall at water depths down to 70 m. Subsequently, 40 stations were studied at three-month intervals. During the summer of 1978, the widest polluted sublittoral areas were localised in the Bays of Morlaix and Lannion down to depths of about 30 m and even deeper in some places. In these two bays, the distribution of hydrocarbons showed: (1) deposition near the coasts hit by the slicks, mostly after adsorption by fine sedimentary particles; (2) redistribution of particulate and adsorbed oil towards low hydrodynamic energy areas favourable to deposition of fine particles. In autumn and winter, the coarse sediments became progressively less polluted. Oil, often in significant quantities, remained or was reconcentrated in the fine sediments of the inner bay parts. Consequently, the hydrodynamic structure ot the area (waves, tidal currents) has played an important role in the deposition and redistribution of the hydrocarbons on sublittoral bottoms.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1433-4909
    Keywords: Key words Heat-shock protein ; Barophile ; Thermococcus barophilus ; Pressure ; Hyperthermophile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The whole-cell protein inventory of the deep-sea barophilic hyperthermophile Thermococcus barophilus was examined by one-dimensional SDS gradient gel electrophoresis when grown under different pressure conditions at 85°C (T opt). One protein (P60) with a molecular mass of approximately 60 kDa was prominent at low pressures (0.3 MPa hydrostatic pressure and 0.1 MPa atmospheric pressure) but not at deep-sea pressures (10, 30, and 40 MPa). About 17 amino acids were sequenced from the N-terminal end of the protein. Sequence homology analysis in the GenBank database showed that P60 most closely resembled heat-shock proteins in some sulfur-metabolizing Archaea. A high degree of amino acid identity (81%–93%) to thermosome subunits in Thermococcales strains was found. Another protein (P35) with molecular mass of approximately 35.5 kDa was induced at 40 MPa hydrostatic pressure but not under low-pressure conditions. No amino acid sequence homology was found for this protein when the 40 amino acids from the N-terminal end were compared with homologous regions of proteins from databases. A PTk diagram was generated for T. barophilus. The results suggest that P habitat is about 35 MPa, which corresponds to the in situ pressure where the strain was obtained.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Normal CBA mouse spleen cells were specifically depleted of cells spontaneously reacting to pigeon erythrocytes (PRBC) by two methods, the first allows specific depletion of anti-PRBC thymus derived (T) rosette forming cells (RFC) whereas the second depletes bone marrow derived (B) anti-PRBC hemolytic plaque forming cells (PFC). Depleted populations transferred into lethally irradiated syngeneic recipients and stimulated with PRBC failed to develop any significant response but they normally responded to a stimulation with sheep erythrocytes (SRBC). When spleen cells were taken from mice skin painted with picryl (trinitrophenyl: TNP) chloride 12 days before and the recipients were challenged in the same way and stimulated with PRBC, they become capable of producing a definite response to this antigen. Moreover in these animals, a consistent although low number of cells was found, which simultaneously reacted to both native PRBC and TNP conjugated SRBC. These findings show that unrelated delayed hypersensitivity reactions promote the immunological recovery of specifically depleted cell populations.
    Type of Medium: Electronic Resource
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