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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 466 (1986), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 177 (1956), S. 95-96 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The deoxyribonucleic acid separated from Nostoc muscorum gives a positive test with the Dische reagent, indicating the presence of deoxypentose-sugar. Chromatographic analysis of the hydrolysate according to the procedure of Wyatt2 revealed only three spots of bases, corresponding to guanine, ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 183 (1959), S. 1824-1825 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Seeds of Avena sativa var. N.P.I and Oryza sativa var. Rupsail were used. The seeds were germinated in the dark with occasional red light for 3 days. The coleoptiles were then decapitated at 2-3 mm. and floated on water for 2 hr. to free them from any endogenous auxin. After a further decapitation ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 181 (1958), S. 1219-1220 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Seven-day old cultures of Chlorella pyrenoidosa and Sce ±edesmus bliquus were centrifuged down at 12 C. in an MSE centrifuge and resuspended in 10 ml. phosphate buffer of pH. 6-8. The cells were then incubated with different concentrations of thioctic acid and 30 JJLC. of sodium ...
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 186 (1960), S. 238-239 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Freshly harvested cells grown in a continuous culture chamber1 were used for isolating ribonucleic acid from Anacystis nidulans. It was found that the method of Ogur and Rosen2 was most suitable. Ribonucleic acid constitutes 0 -37-0 -50 per cent of the fresh weight of the cells. Ribonucleic acid ...
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 188 (1960), S. 1029-1030 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The deoxyribonueleic acid from Anacystis nidulans, Chlorella ellipsoidea and sperm (obtained from Nutritional Biochemical Corporation, Cleveland^ Ohio) was degraded with deoxyribonuclease in the following way. 48 mgm. deoxyribonueleic acid, 1.28 mgm. deoxyribonuclease in 0.003 M magnesium sulphate ...
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Crystal violet from Gram stained S. aureus can be extracted completely by 95% ethanol if the stained bacteria is pre-treated with dilute sodium thiosulphate solution. Thiosulphate removes iodine form the cell component-dye-iodine complex instantaneously and renders the dye extractable by the differentiating medium. 1∶1 alcoholic solutions of aniline, dimethyl aniline, nitro-benzene, benzene, toluene or xylol can also extract the color from the stained S. aureus; the extraction with the first three solvents is almost exhaustive while with the latter solvents extraction is appreciable but incomplete. These solvents can form charge-transfer complexes with iodine. The findings indicate that the stability of the cell component-dye-iodine complex determines the Gram-character of the cell. A model hasbeen presented for the Gram cell component-dye-iodine complex.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 16 (1968), S. 150-153 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Extraction of lipid by chloroform-methanol from Escherichia coli induces Gram positivity in the cells of this bacterium. It has been observed that approximately 1.5 times more lipid is extracted from Escherichia coli than from Staphylococcus aureus by this treatment. An increase of 1.5–1.8 fold in the retention of dye has been evident in the case of S. aureus where as this was 70–90 fold in the case of E. coli, which is 1/4th of the normal retention by S. aureus. The more lipid present in the cell wall or cell membrane, the less retention of dye seems to be exhibited by the bacteria. This also substantiates our model for Gram reaction presented in a previous communication.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 18 (1969), S. 202-209 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. Metachromasia with toluidine blue could be induced by inositol hexaphosphate, the smallest chromotrope molecule so far reported. 2. The metachromatic compound, pink in color, and distinct from orthochromatic dye, could be separated by centrifugation. This complex could as well be decomposed by dil. HCl. 3. The stoichiometry of the metachromatic complex has been studied and the molar ratio of dye: phytate has been shown to vary with varying proportions of dye and phytate used in the reaction mixture and a maximum value of 10 could be obtained suggesting a steric hindrance for the attachment of dye to the phytate-molecule. 4. The divalent cations like, Ba++, Mg++, and Mn++ interfered with the formation of metachromatic complex, possibly competing with the dyes for the anionic sites of the phytate molecule.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 10 (1967), S. 261-265 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Treatment with a solution of the detergent cetyl pyridinium chloride develops gram positivity in E. coli as is evident from the increased retention of crystal violet by the bacteria. Since cetyl pyridinium chloride has been found to remove larger quantity of lipid from E. coli than from the normal gram positive bacteria S. aureus, it is reasonable to think that lipid content in a bacteria may have an important role in determining the gram character of it. Gram negative bacteria generally contain more lipid than the gram positive ones.
    Type of Medium: Electronic Resource
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