Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Cytophotometric determination of nuclear size and DNA distribution in different hyperfunctioning thyroid lesions (1982)
    Springer
    Virchows Archiv 398 (1982), S. 129-137 
    Details
    ISSN: 1432-2307
    Keywords: Thyroid disease ; Feulgen-DNA ; Scanning microdensitometry ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Nuclear enlargement in hyperfunctioning thyroid lesions which has been found in earlier cytophotometric studies is also one of the criteria in the subjective histological estimation of thyroid function. Histopathological assessment is, however, often unreliable. In the present study stage scanning cytophotometric measurements in Feulgen-stained tissue sections were used to determine the nuclear changes encountered in non-toxic and toxic nodular goitre, and in toxic diffuse goitre. To ensure optimal selection of specimens for measurements autoradiography was used. Specimens of toxic diffuse goitre invariably had enlarged nuclei, but no difference was found between nodules in non-toxic and toxic nodular goitre. In fact, the same nuclear area was found in hot nodules, warm nodules and perinodular tissue in non-toxic nodular goitre, and in hot nodules in toxic nodular goitre. Thus there are lesions with clear-cut clinical, biochemical, and autoradiographic hyperfunction that do not have enlarged nuclei. Against this background it is possible that the nuclear enlargement present in toxic diffuse goitre reflects the disorder in itself and not the hyperfunctioning state. Hyperdiploid cell nuclei were found in all cases of toxic diffuse goitre and in a higher precentage than in the other lesions. It was not possible to distinguish nontoxic and toxic nodular goitre on this basis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00618864
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    A comparison of static cytofluorometry and flow cytometry for the estimation of ploidy and DNA replication in human breast cancer (1986)
    Springer
    Breast cancer research and treatment 7 (1986), S. 15-22 
    Details
    ISSN: 1573-7217
    Keywords: breast cancer ; DNA ; flow cytometry ; ploidy ; replication ; static cytofluorometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 332 primary invasive breast carcinomas were analysed by static cytofluorometry and flow cytometry. The ploidy distributions were similar, and 54% of the tumors were judged DNA aneuploid by both methods. The coefficient of variation of the G0–G1 peaks ranged from 2.0 to 8% with both techniques, but the mean was somewhat lower with flow cytometry — 4.1%, compared to 4.9% for the static measurements. The proportion of S-phase cells was possible to estimate from 80% of the flow histograms and 70% of the static histograms. S-phase was not estimated from the static histograms if less than 150 tumor cells were measured. With 160 tumors S-phase was measured by both methods. The range was 0 to 27% with the static measurements and 0.7 to 25% with flow cytometry. Corresponding mean values were 7.6% and 8.2%, which are similar to thymidine labeling index results with breast cancers reported in some studies. A close correlation was obtained (r = 0.927) comparing S-phase fractions estimated from aneuploid tumors with flow cytometry and static cytofluorometry if more than 200 cells were measured with the latter. The proportion of S-phase cells was significantly lower for the diploid tumors. We conclude that both techniques can be useful for the estimation of DNA ploidy and replication in human breast cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01886731
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...