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  • 1
    Electronic Resource
    Electronic Resource
    Helicobacter pylori in the Oral Cavity: (2000)
    Springer
    Digestive diseases and sciences 45 (2000), S. 2162-2167 
    Details
    ISSN: 1573-2568
    Keywords: Helicobacter pylori ; transmission ; oral cavity ; saliva ; PCR ; DNA sequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To test the hypothesis that Helicobacter pylori may be transmitted by the oral–oral route, we applied nested PCR and DNA sequencing to detect and analyze H. pylori DNA in the oral cavity of 20 adult patients undergoing endoscopy. Dental plaques of molars, premolars, and incisors and saliva were collected. Additional paraffin-embedded gastric biopsies were analyzed in four patients. Two sets of highly sensitive and specific primers, EHC-U/EHC-L and ET5-U/ET-5L directed to a 860-bp fragment of H. pylori DNA, were used in the nested PCR. Eight patients had an active infection in the stomach determined with the [13C]urea breath test and the other 12 were negative. Nested PCR showed that all 20 subjects (100%) were positive for H. pylori in the oral cavity. DNA sequencing demonstrated that all tested PCR products of the expected size from the oral samples have more than 97% identity with that from H. pylori type strain ATCC 43629. However, sequences differed in oral samples from different subjects as well as between different oral locations and gastric biopsies within the same individuals. In conclusion, the oral cavity may be a permanent reservoir for H. pylori and can harbor multiple H. pylori strains at the same time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026636519241
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Helicobacterpylori in Dental Plaque (A Comparison of Different PCR Primer Sets) (1999)
    Springer
    Digestive diseases and sciences 44 (1999), S. 479-484 
    Details
    ISSN: 1573-2568
    Keywords: Helicobacterpylori ; PCR ; DENTAL PLAQUE ; TRANSMISSION
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study was designed to compare differentprimer sets for PCR analysis of H. pylori in the sameseries of 40 dental plaque samples. Three pairs ofprimers, HPU1/HPU2, HP1/HP2, and EHC-U/EHC-L, directed to the urease A gene, 16S rRNA gene, or 860-bpDNA of H. pylori, respectively, were used. Our resultsdemonstrate that EHC-L/EHC-U were moRespecific andsensitive for H. pylori added to saliva or dental plaque than HPU1/HPU2 and HP1/HP2. Thedetection rates for H. pylori DNA in dental plaquesamples from randomly selected adult patients from theDental Clinic of the University of Ulm were 26.5% (9/34) for HPU1/HPU2, 78.9% (30/38) for HP1/HP2, and100% (40/40) for EHC-U/EHC-L (P 〈 0.001). Nested PCRusing primers directed to the 860-bp DNA of H. pylorifurThe r confirmed the presence of H. pylori DNA (40/40) in all these samples. Our resultsindicate that primers EHC-U/EHC-L are to be recommendedfor PCR detection of H. pylori in the oralcavity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026680618122
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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