ISSN:
1432-1424
Keywords:
Arachidonate
;
Palmitate
;
Human red cell membrane
;
Equilibrium dissociation constant
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Human red cell membrane bindings of arachidonate and palmitate at pH 7.3 are investigated at temperatures between 0 and 38°C by equilibrating ghosts with the long-chain fatty acids bound to bovine serum albumin in molar ratios (v) within the physiological range (〈1.7). Linearized relations of ghost uptakes and fatty acid monomer concentrations in buffer provide estimates of the binding capacities and corresponding equilibrium dissociation constants (K dm ). The temperature-independent arachidonate binding capacity, 5.5 ± 0.5 nmol g−1 packed ghosts, is approximately fivefold smaller than that of palmitate, 26.6 ± 2.0 nmol g−1. While K dm of arachidonate binding 5.1 ± 0.5 nm is temperature independent, K dm of palmitate increases with temperature from 3.7 nm at 0°C to 12.7 nm at 38°C. The large difference in binding capacities suggests the presence of at least two different fatty acid binding domains in human red cell membranes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00233388
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