ISSN:
1600-0625
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract In this study we show the expression of the newly identified carbohydrate ligand. sialosyl-Lex on Langerhans cells. The receptor Tor sialosyl-Lex is the endothelial leukocyte adhesion molecule-1 (ELAM-1) present on activated endothelial cells. Using How cytometry. Langerhans cells were selected due to positivity for an antibody against CD la and low orthogonal light scatter. The CD la antigen stained by the OK T6 antibody is considered a maturational marker of Langerhans cells in agreement with the specific labeling of dendritic cells in the epithelium only. Double immunostaining (OKT6/anti-sialosyl-Lex) using flow cytometry and immunohistochemistry demonstrated that almost all OKT6-positive cells in normal stratified epithelium expressed sialosyl-Lex. Conversely, by immunohistochemistry of oral epithelium with acute inflammation, additional dendritic cells negative for OKT6 were found to express sialosyl-Lex In addition, sialosyl-Lex-positive but not OKT6-positive dendritic cells were found in the submucosa. These findings indicate that the carbohydrate antigen sialosyl-Lex is expressed earlier than the CD la antigen in the maturation of the Langerhans cell lineage. Future studies should aim at investigating the importance of adhesion between sialosyl Lex and ELAM-1 in epithelial recruitment of Langerhans cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0625.1992.tb00082.x
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