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  • 1
    Electronic Resource
    Electronic Resource
    Short chain fatty acids present in periodontal pockets may play a role in human periodontal diseases (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 22 (1987), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1987.tb01565.x
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  • 2
    Electronic Resource
    Electronic Resource
    Gas-liquid chromatography of the gingival fluid as an aid in periodontal diagnosis (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 20 (1985), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: There is increasing evidence that rod-shaped Gram negative anaerobic bacteria, as those belonging to black-pigmented Bacteroides, may play a role in the pathogenesis of periodontal diseases. Anaerobic bacteria are known to produce, as end-products of their metabolism, several volatile fatty acids, whose detection in clinical specimens by direct gas liquid chromatography (GLC) has been usefully utilized to demonstrate their presence in the infected site. The results obtained analyzing by GLC the gingival fluid collected from patients suffering from periodontal diseases are reported here. A good statistical correlation of the GLC profile has been found with the gingival index and the colonization by gram-negative bacteria at each selected site.The data obtained strongly suggest that butyric acid may be considered a reliable marker of the severity of the disease, and its quantitative changes may be useful in following the clinical course of the process. The data correlate well with the biological activities of butyric acid, which have been reported to be abundant in plaque and to exert a cytotoxic activity on a variety of cells including gingival fibroblasts.No correlation has been found between gingival fluid flow rate and fatty acids profile in GLC. It can be concluded that GLC measures the overall metabolic activity of the bacterial population colonizing the subgingival area, while the rate of gingival fluid flow reflects the degree of the inflammatory response of the host. The combined use of the two laboratory tests should provide the clinician with more quantitative and reproducible parameters for monitoring the disease activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1985.tb00827.x
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  • 3
    Electronic Resource
    Electronic Resource
    Impact of hepatitis C virus infection on clinical features, quality of life and survival of patients with lymphoplasmacytoid lymphoma/immunocytoma (1998)
    Springer
    Annals of oncology 9 (1998), S. 499-504 
    Details
    ISSN: 1569-8041
    Keywords: clinical features ; HCV infection ; lymphoplasmacytoid lymphoma/immunocytoma ; quality of life
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: The non-Hodgkin‘s lymphoma (NHL) subgroup most frequentlyassociated with hepatitis C virus (HCV) infection is the lymphoplasmacytoidlymphoma/immunocytoma (Lp-Ic). We have assessed the impact of the infectionon the clinical features, quality of life and survival of HCV+ve Lp-Icpatients as compared to its impact in HCV−ve patients. Patients and methods: Seventy patients with Lp-Ic consecutively observedover a six-year period were studied. Clinical, virological andhistopathological features were recorded at diagnosis. Quality of life wasassessed using a scoring system including disease-related symptoms,performance status, working ability, hospital admissions and therapiesrequired. Results: Eighteen patients (26%) with HCV infection wereidentified. Significant differences between those patients and theHCV−ve group included number of symptomatic patients, Hb levels, serumprotein levels, entity of the IgM monoclonal component, number of patientswith cryoglobulins and with organ (liver, kidney) involvement, and entityand pattern of bone marrow infiltration. Survival rates were similar (P =0.8383), but the quality-of-life score was significantly worse for theHCV+ve patients (P = 0.002). All anti-HCV Ab+ve patients tested positive forHCV RNA; genotype 2ac was detected in a significant proportion of cases. Conclusions: This study confirms that HCV infection is present in aboutone-third of patients with Lp-Ic. HCV infection does not seem to affect theoverall survival of patients with Lp-Ic, but it affects the clinicalexpression of the disease, so that the overall quality of life of HCV+vepatients is significantly worse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008265804550
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  • 4
    Electronic Resource
    Electronic Resource
    Vertical transmission of hepatitis C virus in low-risk pregnant women (1996)
    Springer
    European journal of clinical microbiology & infectious diseases 15 (1996), S. 116-120 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To assess the prevalence of hepatitis C virus (HCV) infection in pregnant women and the rate of vertical transmission in infected mothers belonging to a low-risk group, 1,388 women were tested for HCV antibody at delivery. Twenty-five anti-HCV-positive women with no apparent source of HCV exposure were recruited. A reverse transcriptasepolymerase chain reaction (RT-PCR) and a new quantitative branched DNA-based signal amplification assay (bDNA) were used to detect HCV RNA. The rate of anti-HCV positivity in pregnant women was 2.5% (36 of 1,388). Of the 25 cohort mothers, 18 (72%) were positive for HCV RNA by RT-PCR, 13 of whom were also positive by the bDNA assay (sensitivity 72.2%). Of the 25 infants of low-risk mothers tested at birth, 22 were anti-HCV positive, two were weakly reactive, one was negative, and none was viremic. Neither active humoral immunoresponse nor HCV RNA was detected in any of the infants over a period of 12 months. These data suggest a relatively high prevalence of anti-HCV in unselected pregnant women and a poor efficiency of vertical transmission of HCV in a low-risk population, irrespective of the viral burden of the mother-to-be.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01591483
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  • 5
    Electronic Resource
    Electronic Resource
    Rapid polymerase chain reaction method for specific detection of toxigenicClostridium difficile (1995)
    Springer
    European journal of clinical microbiology & infectious diseases 14 (1995), S. 716-719 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A rapid polymerase chain reaction (PCR) method for detection of toxigenicClostridium difficile directly from fecal samples by amplification of toxin Agene fragments was investigated. The technique was applied to monitor the spread of the microorganism in a long-term care ward with a relatively high incidence of overt episodes of diarrhea. The PCR approach has several advantages over traditional methods, rapidly allowing the specific detection of toxigenicClostridium difficile strains from stool samples in both symptomatic and asymptomatic subjects with toxigenic strains. This PCR method allows early detection of toxigenicClostridium difficile and could thus represent a powerful tool for the surveillance of epidemics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01690883
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  • 6
    Electronic Resource
    Electronic Resource
    Evaluation of an automated system for identification of anaerobic bacteria (1994)
    Springer
    European journal of clinical microbiology & infectious diseases 13 (1994), S. 135-141 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A fully automated computer-assisted system (ATB system, bioMérieux, France) which uses disposable microenzymatic panels was evaluated for its ability to identify 215 strains of anaerobic bacteria (clinical isolates and reference strains). All strains were examined using conventional identification protocols and by gas chromatographic analysis of short-chain fatty acids. Automated reading of Rapid ID32A test kits (bio-Mérieux, France) by the ATB system gave correct identification for 195 strains (90.7 %): 92.25 % of gram-negative anaerobes (116 strains) and 89 % of gram-positive anaerobes (99 strains) were correctly identified. Twelve strains (5.6 %) were incorrectly identified and 8 strains (3.7 %) were not identified by the system. For some strains in theBacteroides fragilis group, forClostridium difficile and for theFusobacterium genus, additional tests suggested by the ATB software were necessary to reach a final identification at the species or genus level. On the basis of the high incidence of correct identifications and the comparison of these results with those obtained previously using other commercially available kits, the ATB system was found to be a reliable method for identification of anaerobic bacteria in clinical laboratories.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01982186
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