ISSN:
1573-5168
Keywords:
salmonid
;
sperm
;
seminal fluid
;
membrane
;
protein
;
electrophoresis
;
immunoblot
;
lipoprotein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The protein composition of seminal fluid, blood serum, sperm plasma membrane and flagellum of rainbow trout were analysed by SDS-polyacrylamide gel electrophoresis. Immunological identity between proteins of the 2 fluids and sperm components was studied using crossed immunoelectrophoresis, rocket immunoelectrophoresis and immunoblotting. Results indicate that many seminal proteins are antigenically-related to serum proteins, proteins of sperm origin are present in seminal fluid in varying amounts, depending on the animals and sampling time, and several serum-like seminal proteins are bound to spermatozoa. Lipoproteins were isolated from seminal fluid (mean level: 33 μg/ml) and characterized. They were identified as being HDL-like lipoproteins. A possible physiological role is proposed for these seminal lipoproteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00003405
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