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Growth of mouse fibroblast-like cells in an autoclavable medium (1968)

Orlando, M. D. ; Bowersox, O. C. ; Riley, J. M.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 10 (1968), S. 61-67

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: A relatively simple, autoclavable medium was developed that would support the growth of the mouse fibroblast (MFL) cell line in suspension culture. This medium was prepared in three stages with decreasing quantities of serum. As the serum was reduced from 5% to none, the amount of Bacto-Peptone was increased from none to 0.5%. The reduction and finally elimination of serum did not affect proliferation adversely, but actually seemed to produce more rapid growth and higher levels of cell population.

Additional Material: 3 Tab.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260100105

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