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  • 1
    Electronic Resource
    Electronic Resource
    Control of thiophene accumulation in calli of two Tagetes species (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 76 (1989), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The relation between root differentiation and accumulation of biocidal thiophenes was studied in stem calli of two Tagetes species. Disorganized tissues of T. erecta were low in thiophene production. A sharp rise in thiophene content coincided with the emergence of roots on the calli. Root regeneration and the amount of thiophenes produced were found to be quantitatively related. Callus tissues of T. minuta did not differentiate into organs. Nevertheless, they accumulated thiophenes throughout the incubation period. Light at 12 W m- stimulated thiophene production in T. erecta without having an apparent effect on root regeneration. In T. minuta even low irradiance levels (2 W m∼2) strongly inhibited thiophene accumulation. Under favorable conditions thiophene concentrations in calli of both species were comparable to or somewhat lower than the levels in roots on the plants and in excised, cultured roots of T. erecta. We conclude that in calli of T. erecta thiophene accumulation is coupled to root regeneration whereas a different control mechanism allowing for accumulation in disorganized tissues is operative in T. minuta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1989.tb05633.x
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of amino compounds on nitrate utilization by roots of dwarf bean
    Amsterdam : Elsevier
    Phytochemistry 24 (1985), S. 653-657 
    Details
    ISSN: 0031-9422
    Keywords: Papilionaceae ; Phaseolus vulgaris ; amino compounds. ; dwarf bean ; nitrate reductase activity ; nitrate uptake
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)84871-2
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  • 3
    Electronic Resource
    Electronic Resource
    Secondary metabolism in plant cell cultures - P. Morris, A.H. Scragg, A. Stafford and M.W. Fowler (Editors). Cambridge University Press, Cambridge, U.K., 1986, 266 pp., £ 22.50, 37.50. ISBN 0 521 32889 6
    Amsterdam : Elsevier
    Scientia Horticulturae 35 (1988), S. 157-159 
    Details
    ISSN: 0304-4238
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0304-4238(88)90047-7
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of aggregate size in cell cultures of Tagetes patula on thiophene production and cell growth (1989)
    Springer
    Applied microbiology and biotechnology 30 (1989), S. 18-25 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effect of the size of Tagetes patula (marigolds) cell aggregates on growth and thiophene production in MS-medium was studied. A heterogeneous aggregate suspension was aseptically divided into 7 fractions, each with a defined aggregate diameter range, with subsequent inoculation of the fractions into MS growth medium. Growth occurred in all aggregate fractions and thiophene production increased with increasing aggregate diameter starting at about 3 mm, an effect possibly due to an increasing lack of oxygen in the aggregate centre. Calculations of oxygen concentration profiles in the aggregates showed namely, that the critical aggregate diameter where the oxygen concentration in the aggregate centre becomes very low, is about 3 mm. Aggregates with a diameter exceeding 1.2 cm showed a decreased thiophene production, however, these aggregates were hollow. The thiophenes produced mainly consisted of 5-(4-hydroxy-1-butenyl)1-2,2′-bithienyl, which was excreted into the medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00255991
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  • 5
    Electronic Resource
    Electronic Resource
    Thiophene accumulation in relation to morphology in roots of Tagetes patula (1989)
    Springer
    Planta 179 (1989), S. 43-50 
    Details
    ISSN: 1432-2048
    Keywords: Agrobacterium ; Auxin and thiophene in roots ; Root culture ; Root morphology ; Tagetes ; Thiophene ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Roots of marigold (Tagetes patula L.) accumulate thiophenes, heterocyclic sulfurous compounds with strong biocidal activity. In detached roots cultured in vitro, the thiophene content was 5 μmol·(g fresh weight)-1 which is 25-times higher than in roots attached to the plant. In roots derived from tissues transformed by Agrobacterium tumefaciens and A. rhizogenes, the morphology and thiophene content varied with the bacterial strain used. Transformation stimulated the elongation of the root tips and the formation of lateral roots but lowered the thiophene level to 20–50% relative to the concentration in untransformed detached roots. A negative correlation was found between the number of laterals in a root system and the thiophene content. Extensive branching and a decrease in thiophene accumulation was evoked in untransformed roots by indole-3-acetic acid (1–10 μmol·l-1) added to the medium. Within the roots, the highest thiophene concentrations were found in the tips. The results indicate that auxin directly or indirectly plays a role in the regulation of the thiophene level in root tips.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395769
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    Paper (German National Licenses)
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