ISSN:
1617-4623
Keywords:
Maize
;
Alcohol dehydrogenase
;
Somaclonal variation
;
Mutation
;
Gene sequence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Plants regenerated from tissue cultures of maize were screened for variants of ADH1 and ADH2. Root extracts of 645 primary regenerant plants were tested, and one stable mutant of Adh1 was detected. The mutant gene (Adh1-Usv) produces a functional enzyme with a slower electrophoretic mobility than that of the progenitor Adh1-S allele, and is stably transmitted to progeny. The mutant was not present among four other plants derived from the same immature embryo, and therefore arose as a consequence of the culture procedure. The gene of Adh1-Usv was cloned and sequenced. A single base change in exon 6 was the only alteration found in the gene sequence. This would translate in the polypeptide sequence to a valine residue substituting for a glutamic acid residue, resulting in the loss of a negative charge and the production of a protein with slower electrophoretic mobility.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00331642
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