ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A 70-fold purification of a commercial pectinase from Aspergillus niger was achieved by gel filtration, ion exchange and hydrophobic chromatographic columns employed in sequence. An apparent Km of 4.5 ± 10-5M and a VM of 1.9 mM/minmg were obtained. The purified preparation reached a higher degree of purification than those reported in the literature but was not electrophoretically homogeneous. The preparation was an endo-polygalacturonase in nature and preferred high molecular weight pectates as substrates. The pH optimum of 4.4 was not affected by the size of substrates. Hydrophobic chromatography is a useful tool for the preparation of pectic enzymes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1982.tb12954.x
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