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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of applied ichthyology 5 (1989), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In einem vergleichenden Fütterungsversuch wurden Coregonenlarven mit Nauplien des Artemia-Stammes Great Salt Lake/Utah beziehungsweise San Francisco Bay gefüttert. Trotz sehr geringer Mortalitäten in beiden Fütterungsansätzen konnten die Gruppen anhand der Ultrastruktur des Darmepithels eindeutig unterschieden werden. Coregonenlarven, die mit Nauplien des Artemut-Stammes Great Salt Lake gefüttert worden waren, hatten große Mengen Bakterien im Darm und zeigten Schädigungen der Darmschleimhaut von unterschiedlichem Schweregrad. Die Ergebnisse wurden im Vergleich zu Darmepithelveränderungen bei Fischen diskutiert, die anderweitig verursacht wurden, sowie zu bakteriellen Darminfektionen bei Säugetieren.〈section xml:id="abs1-2"〉〈title type="main"〉SummaryDamage to the intestinal epithelium of coregonid larvae (Coregonus fera) due to Artemia feeding: A bacterial infectionIn a comparative feeding experiment coregonid larvae were fed with nauplii of Artemia strain Great Salt Lake/Utah and San Francisco Bay, respectively. Though very low mortalities were observed the different feeding groups can be distinguished by means of the ultrastructure of the intestinal epithelium. Coregonid larvae fed with nauplii of Artemia strain Great Salt Lake/Utah had a lot of bacteria in the intestine and showed damage to the intestinal epithelium in different extent. The results were discussed in comparison with alterations of the intestinal epithelium of fish caused by other factors as well as with bacteria! intestinal infections in mammals.〈section xml:id="abs1-3"〉〈title type="main"〉Résumé Lésion de l'épithélium intestinal des larves de corégones (Coregonus fera) causée par une alimentation sà base d'artémia: une infection bactérielleDans une expérience comparative des larves de corégones ont été nourries avec des nauplius de la souche Great Salt Lake/Utah et San Francisco Bay d'artémia, respectivement. En dépit d'une faible mortalité les deux groupes d'alimentation pouvaient être clairement différenciés sà l'aide de l'ultrastructure de l'épithélium intestinal. Les larves de corégones nourris avec des nauplius de la souche Great Salt Lake/Utah d'artémia avaient beaucoup de bactéries dans l'intestin et montraient des lésions sur la muqueuse intestinale sà des degrés différents. Les résultats ont été discutés et comparés avec les altérations de l'épithélium intestinal chez les poissons causées par d'autres facteurs et aussi avec les infections bactérielles intestinales chez les mammifères.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1420-9055
    Keywords: Key words: Environment, sewage plant, effluent, water quality, brown trout, health.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract: The objective of the present study was to elucidate the influence of treated wastewater from a sewage plant on the state of health of brown trout (Salmo trutta fario). Trout were kept in two cages in a moderately polluted river at a site where a sewage plant discharges. River water only flowed through one cage (river water only (RWO) trout), while treated sewage water was added to the river water (final concentration 5 %) in a second cage (river and wastewater (RWW) trout). As a reference, fish were kept in tap water (tap water reference (TWR) trout). ¶After 66 days the mortality rate in group RWW was 87 %, in group RWO 29% and in group TWR 0 %. In RWW and RWO trout the infectious agent of furunculosis (A. salmonicida) and additionally in RWW another bacterial species (A. hydrophila) were isolated. Parasites were found in fish from both cages, whereby there were differences in species composition and degree of infestation between the two groups. Only in RWW trout were ulcerations of the upper and lower jaw found, together with significantly more severe histological liver alterations and a higher lactatdehydrogenase (LDH) enzyme level in the blood.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0878
    Keywords: Enteroendocrine cells ; Immunohistochemistry ; Bombesin ; Enkephalin ; Gastrin/CCK ; 5-HT ; Neuropeptide Y ; Neurotensin ; Substance P ; VIP ; Intestine ; Starvation ; Leuciscus idus melanotus ; Poecilia reticulata (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Endocrine cells containing bombesin-, enkephalin-, gastrin/CCK-, 5-HT-, and substance P-like material were demonstrated in the alimentary tract of Poecilia reticulata and Leuciscus idus melanotus. Endocrine cells with neuropeptide-Y-like immunoreactivity were found only in P. reticulata, those with VIP-like immunoreactivity only in L. idus melanotus. Gut nerves showing bombesin-, G/CCK-5-HT-, neurotensin-, substance P-and VIP-like immunoreactivity were observed in both species investigated, enkephalin- and neuropeptide Y-like immunoreactivity in P. reticulata alone. The distribution and amount of endocrine cells and nerves along the gut as visualized with the appropriate antisera varied in both teleosts. Histologically, the intestinal tract of these stomachless fish can be divided into three regions. A large number of endocrine cells with VIP-like immunoreactivity was noted in the rectum of L. idus melanotus. Endocrine cells containing bombesin-, enkepha-lin- and substance P-like material were found only in intestinal parts I and II in L. idus melanotus. Neuropeptide Y-like immunoreactivity was absent from intestinal part I of P. reticulata. The influence of starvation on the immunoreactivity of nerves and enteroendocrine cells in the teleost intestine was examined. After a starvation period of more than 6 weeks, no alterations were observed either in the appearance or amount of nerve and endocrine cell immunoreactivity.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Metallothionein, a biomarker of exposure and toxicity of heavy metals, has been detected in the gills of brown trout (Salmo trutta fario L.) and rainbow trout (Oncorhynchus mykiss Richardson) by means of immunohistochemistry. A very prominent labelling of chloride cells was found after exposure to diluted sewage plant effluents. No significant increase was observed in either the number of labelled cells or their labelling intensity after exposure to water of a polluted river compared to fish kept in tap water. These results do not correlate with findings of a histopathological study, suggesting that the metal levels at the sewage treatment plant were too low to produce gross histopathology. A comparison between the species indicated that the rainbow trout showed a generally higher metallothionein expression than the brown trout.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular histology 29 (1997), S. 893-899 
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In order to characterize the glycoconjugate residues in skin and gills of the adult rainbow trout, the binding pattern of five biotinylated lectins with different carbohydrate specificities was examined. In the skin, mucous cells revealed binding sites for PNA and SBA; filament-containing cells were additionally labelled with Con A. However, the basal cell layer showed no reaction. In the gill, subpopulations of mucous cells reacted with Con A, PNA, SBA and UEA-I. This broader spectrum of glycoconjugates in gill mucous cells compared with the epidermal mucous cells could point to the additional function of gill mucus in ion and osmoregulation. Lectin binding sites were less common in the respiratory epithelial cells of the secondary lamellae than in those of the primary lamellae. Chloride cells revealed mannose, galactose and fucose residues. Immature chloride cells, as indicated by a comparison with Na+/K+ ATPase immunolabelling, reacted with Con A; subpopulations of them reacted with PNA, SBA and UEA-I. The results form the basis for further investigations in which these cell populations can be analysed under different environmental conditions
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5141
    Keywords: discriminant analysis ; integrated responses ; sewage effects ; trout health
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The impact of wastewater effluent from a sewage treatment works (STW) on the health of brown trout held in cages and wild brown trout in a river was investigated. Biochemical, histological, and organismal responses as well as parasite abundances were monitored and then analyzed using multivariate analyses. Stress responses in trout induced by the water quality of the river upstream of the STW were enhanced by the discharge of the STW. For caged fish, the serum chemistry values alkaline phosphatase, blood urea nitrogen, and creatinine, as well as histological indices of gills and liver, were most effective at distinguishing among fish held in river water, a mixture of river water and wastewater, and tap water. For wild fish, total protein, histological liver alterations and abundance of two parasites (sessile peritrichia, Sphaerospora sp.) were the most indicative parameters for discriminating the health of fish between sites upstream and downstream of the STW. Considering the relationships between the measured parameters concurrently, the multivariate discriminant analysis is an effective method to evaluate which combination of parameters provide the best discrimination between the treatment groups. In contrast to the calculation of group differences based on individual responses, the integrated responses of parameters representing different biological levels lead to a more comprehensive assessment of organismal health and a more accurate distinction in differences between treatment groups.
    Type of Medium: Electronic Resource
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