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  • 1
    Electronic Resource
    Electronic Resource
    Toward a unified genetic map of higher plants, transcending the monocot–dicot divergence (1996)
    [s.l.] : Nature Publishing Group
    Nature genetics 14 (1996), S. 380-382 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Sir — Closely related (confamilial) genera often retain large chromosomal tracts in which gene order is co linear, punctuated by structural mutations such as inversions and translocations1. To explore the possibility that conservation of gene order might extrapolate to more distantly related ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1296-380
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of cDNA clones of genes induced upon transfer of Chlamydomonas reinhardtii cells to low CO2 (1996)
    Springer
    Plant molecular biology 31 (1996), S. 443-448 
    Details
    ISSN: 1573-5028
    Keywords: carbonic anhydrase ; Chlamydomonas ; CO2 concentrating mechanism ; gene regulation by CO2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specific to low CO2-adapting cells. Six classes of low CO2-inducible clones were identified. One class of clone, reported here, represents a novel gene associated with adaptation of cells to air. A second class of clones corresponds to the air-inducible periplasmic carbonic anhydrase I (CAH1). These clones represent genes that respond to the level of CO2 in the environment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021807
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  • 3
    Electronic Resource
    Electronic Resource
    Apical and lateral shoot apex-specific expression is conferred by promoter of the seed storage protein β-phaseolin gene (1993)
    Springer
    Transgenic research 2 (1993), S. 21-28 
    Details
    ISSN: 1573-9368
    Keywords: 5′-deletion ; β-glucuronidase ; histochemical staining ; phloem ; xylem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A previous analysis with deletion mutants of the native β-phaseolin gene demonstrated that removal of a negative element 5′ upstream of−107 permitted phaseolin expression in stem cortex and secondary root (Burowet al., 1992). Here we employed the β-glucuronidase (GUS) reporter gene to visualize, by histochemical staining, the cell type-specificity of phaseolin expression in stem and root, and to understand further the spatial control of the β-phaseolin gene. The 782 bp 5′ upstream promoter and its deletion mutants were fused to the GUS gene, and these chimaeric genes were used to transform tobacco. Histochemical staining for GUS activity demonstrated that phaseolin promoters truncated downstream of −227 conferred cell-type specific expression in internal/external phloem and protoxylem of mature stem. Surprisingly, GUS staining was prominent in both apical and lateral shoot apices of plants that contain the full-length −782 promoter and mutant promoters deleted up to −64. GUS expression was extended to all cell types of shoot tips, including epidermis, cortex, vasculature, procambium and pith. Expression in vasculature of petioles was limited to plants with promoters truncated to −106 and −64. The current results are in agreement with our previous findings with the native phaseolin gene: that the major positive element (−295/−228) is sufficient for seed-specific late-temporal expression of the phaseolin gene. We conclude that the 5′ upstream sequence of the β-phaseolin gene directs spatially- and temporally-controlled gene expression in developing seeds during the reproductive phase, but also confers expression in shoot apices during the vegetative phase of plant development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01977677
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  • 4
    Electronic Resource
    Electronic Resource
    Suppression of phaseolin and lectin in seeds of common bean, Phaseolus vulgaris L.: increased accumulation of 54 kDa polypeptides is not associated with higher seed methionine concentrations (1993)
    Springer
    Molecular genetics and genomics 241 (1993), S. 431-439 
    Details
    ISSN: 1617-4623
    Keywords: Phaseolus vulgaris L ; Common bean ; Methionine ; Seed proteins ; Nutritional value
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Suppression of phaseolin and lectin accumulation in common bean resulted in higher concentrations of bean seed polypeptides with apparent molecular weights of 54 kDa and from 70 to 84 kDa on SDS-polyacrylamide gel electrophoresis. Polypeptides of 54 and 56 kDa segregated as products of different alleles. Genes for the 54/56 kDa bands and phaseolin were estimated to be 26.2±3.7 map units apart. The 54 kDa band phenotype manifested by SDS-PAGE consisted of from one to three polypeptides of 54 kDa MW on 2D gels, and the 56 kDa phenotype consisted of one polypeptide of 56 kDa plus two minor polypeptides of 54-54.5 kDa molecular weight. The pKI of these polypeptides was approximately 5.25. The methionine content of the 54 kDa polypeptides of the cultivar Great Northern Star was 1.6±0.1 g/100 g protein, which was not statistically different from the value (1.5±0.1%) obtained for phaseolin isolated by the same procedure. F2 seeds deficient for phaseolin and lectin contained as much total N per g as wild-type seeds and were not shrunken, but contained 50% more free amino acids. F2 seeds from two of the three populations contained from 8 to 13% less methionine per mg total N.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00284697
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  • 5
    Electronic Resource
    Electronic Resource
    Identification of peanut (Arachis hypogaea L.) RAPD markers diagnostic of root-knot nematode (Meloidogyne arenaria (Neal) Chitwood) resistance (1996)
    Springer
    Molecular breeding 2 (1996), S. 369-379 
    Details
    ISSN: 1572-9788
    Keywords: genetic mapping ; DNA marker-assisted selection ; introgression ; bulked segregant analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract DNA markers linked to a root-knot nematode resistance gene derived from wild peanut species have been identified. The wild diploid peanut accessions K9484 (Arachis batizocoi Krapov. & W. C. Gregory), GKP10017, (A. cardenasii Krapov & W. C. Gregory), and GKP10602 (A. diogoi Hoehne) possess genes for ressitance to Meloidogyne arenaria. These three accessions and A. hypogaea cv. Florunner were crossed to generate the hybrid resistant breeding line TxAg-7. This line was used as donor parent to develop a BC4F2 population segregating for resistance. Three RAPD markers associated with nematode resistance were identified in this population by bulked segregant analysis. Linkage was confirmed by screening 21 segregatingh BC4F2 and 63 BC5F2 single plants. Recombination between marker RKN410 and resistance, and between marker RKN440 and resistance, was estimated to be 5.4±1.9% and 5.8±2.1%, respectively, on a per-generation basis. These two markers identified a resistance gene derived from either A. cardenasii or A. diogoi, and were closely linked to each other. Recombination between a third marker, RKN229, inherited from A. cardenasii or A. diogoi, and resistance was 9.0±3.2% per generation. Markers RKN410 and RKN229 appeared to be linked genetically and flank the same resistance gene. All markers were confirmed by hybridization of cloned or gel-purified marker DNA to blots of PCR-amplified DNA. Pooled data on the segregation of BC5F2 plants was consistent with the presence of one resistance gene in the advanced breeding lines. Different distributions of resistance in the BC5F2 progeny and TxAG-7 suggest the presence of additional resistance genes in TxAG-7.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437915
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