ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
2G-3 is an anti-anti-idiotypic MoAb (Ab3) obtained upon immunization wiih a monoclonal Ab2 (A3B10), which behaves as the “internal image” of CaMBrl. a saccharidic epitope defined by MoAb MBrl (Abl), CaMBrI is expressed on glycoconjugates of the human mammary carcinoma cell line MCF-7 und on normal and neoplastic mammary gland epithelial cells, Abl and Ab3. although exhibiting, in many respects, superimposable paratopic and idiotopic specificities, show a non-identical fine immunoreactivity, since 2G-3 has a preferential reactivity with the saccharidic epitope mounted on glycoproteins. while MBrl reacts with both glycoproteins and glycolipids, V-region sequence analysis has shown that: (i) the VK genes employed belong to different families(VK 1 and VK 10); (ii) the J K2 segment is shared by the two L chains(thus a high degree of homology is observed between VK CDR3s): (iii) the VH genes employed derive from the same family VHIIBJ55K (but show CDR homology only in CDR2): (iv) different JM region genes are employed. These data together with the comparison of deduced secondary structure parameters give further evidence fur the possible production of similar combining sites using different VH and VL germ-line genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1991.tb01559.x
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