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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of a Neutral Aminoacyl-Peptide Hydrolase from Naegleria fowleri1 (1987)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 34 (1987), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An intracellular alpha-aminoacyl-peptide hydrolase (EC 3.4.11.-) from Naegleria fowteri nN68 (ATCC 30894) has been characterized. The enzyme preparation hydrolyzed phenylalanyl-, tyrosyl-, leucyl-, arginyl-, alanyl-, tryptophanyl-, histidyl-, methionyl-, and lysyl-naphthylamide but not benzoylleucyl-, leucylglycyl-, glycylprolylleucyl-, glycyl-, threonyl-, aspartyl-, or glutamyl-naphthylamide. The aminopeptidase activity was inhibited by the cysteine-protease inhibitors—hydroxymercuribenzoate, chloromercurisulfate, and iodoacetate- by the aminopeptidase inhibitors-bestatin and trans-epoxysuccinyl-leucyl-agmatine- by an inhibitor of soluble alanyl aminopeptidase EC 3.4.11.14, puromycin, and by the metalloprotease inhibitor, o-phenanthroline. The exopeptidase activity was not inhibited by the chelator, ethylenediaminetetraacetate, or the serine-protease inhibitor, phenylmethylsulfonylfluoride. The pH optimum of the exopeptidase was between 7.0 and 8.0. Enzyme activity was stable at 55°C for 30 min, but all activity was lost after 15 min at 80°C. Enzyme activity was inhibited by 100 μM HgCI2 and CdCl2 but not by 1 mM CoCl2, CuCl2, MnCl2, NiCl2, FeCl2, or ZnCl2. Enzyme activity was inhibited by 0.1% sodium dodecyl sulfate but not by 0.2% Brij 35, Tween 20, Tween 80, or Triton X-100.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1987.tb03151.x
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  • 2
    Electronic Resource
    Electronic Resource
    Comparison of Naegleria fowleri and Naegleria gruberi Cultivated in the Same Nutrient Medium (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The human pathogenic amoeboflagellate Naegleria fowleri and the nonpathogenic species N. gruberi can be cultivated axenically but usually in different media. Naegleria fowleri 6088 has been adapted to grow in Balamuth H-4 medium, usually used to propagate N. gruberi nB81. and nB81 has been adapted to grow in supplemented Nelson's medium, usually used to propagate N. fowleri. N. gruberi nB81. grown in either medium, enflagellated 135 to 150 min after subculture to non-nutrient amoeba saline, whereas 6088 required 225 min. Naegleria gruberi nB81 grown in either medium was agglutinated by 100 ug concanavalin A/ml, whereas N. fowleri 6088 was not. Naegleria fowleri and N. gruberi grown in Nelson's medium became rounded to a greater extent upon chilling at 5° C and remained rounded longer than Naegleria grown in Balamuth medium. The specificity of the surface antigens was an inherent characteristic of each species and not dependent upon the propagating medium. but Naegleria grown in Nelson's medium was agglutinated more reproducibly and more effectively by antiserum. N. gruberi was somewhat more resistant to acriflavine, actinomycin D, cycloheximide, or tetracycline than N. fowleri, regardless of the culture medium. Naegleria fowleri 6088 grown in Nelson's medium, however, was more resistant to actinomycin D, daunomycin. mithramycin. sulfamethoxazole, or tyrocidine than 6088 grown in Balamuth medium. There are limitations on the validity of comparisons of N. fowleri and N. gruberi based upon cultures grown in different media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1983.tb02936.x
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  • 3
    Electronic Resource
    Electronic Resource
    Cytopathogenicity of Naegleria fowled for Cultured Rat Neuroblastoma Cells (1985)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 32 (1985), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The cytopathogenicity of Naegleria fowleri strain LEE (ATCC-30894) for cultured rat neuroblastoma cells (B-103) has been investigated. Both live N. fowleri amoebae and Naegleria lysates added to 51Cr-labeled B-103 cells caused release of radiolabel, which was dependent upon the ratio of amoebae to target cells or to the lysate concentration. Lysates of N. fowleri strains LEE, NF-66, NF-69, and HB-4 were equally injurious to B-103 target cells whereas lysates of strains 6088 and KUL were less cytotoxic. Highly pathogenic mouse-passaged strain LEE were less cytotoxic than axenically grown amoebae. Maximum cytotoxicity was observed in lysates from amoebae in late exponential or early stationary phase of growth. Cytopathogenicity of lysates was reduced after heating at 44°C for 60 min or at 60°C for 30 min. Cytotoxicity was stable during storage at 4°C or at −20°C for 26 h. Neither live amoebae nor lysates injured B-103 target cells at 4°C. Live amoebae and lysates injured B-103 by a time, temperature, and concentration dependent process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1985.tb03034.x
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  • 4
    Electronic Resource
    Electronic Resource
    Potentially mineralizable nitrogen from organic materials applied to a sandy soil: fitting the one-pool exponential model (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 21 (2005), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Over the last three decades there has been a great increase in the production of waste from urban, industrial and agricultural activity that could be recycled as a source of plant nutrients, and used to enhance soil quality. The use of these materials could partially offset the need for mineral fertilizers, giving both economic and environmental benefits. An incubation experiment was carried out using different organic waste materials applied to a Cambic Arenosol. Air-dried soil was mixed with increasing amounts of composted solid municipal waste, secondary pulp-mill sludge, hornmeal, poultry manure, solid phase from pig slurry, and composted pig manure, resulting in applications equivalent to 0, 40, 80, 120, 160 and 200 kg ha−1 of Kjeldahl nitrogen. The samples were incubated for 244 days under a controlled environment of 24 °C and 60% water holding capacity of the soil. The increasing amounts of waste applied always led to a greater amount of potentially available nitrogen present in the soil/waste mixture. Based on the proportion of their active N fraction, wastes were ranked: poultry manure〉hornmeal〉solid phase from pig slurry〉composted pig manure〉secondary pulp-mill sludge〉composted municipal solid waste. The results were well described by a one-pool exponential mineralization model, and mineral N formation was proportional to the quantity of waste applied. Of the wastes tested, those from animal sources showed greater nitrogen mineralisation. Nitrification was rapid, and concentrations of ammonium nitrogen remained relatively small.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2005.tb00108.x
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  • 5
    Electronic Resource
    Electronic Resource
    Activation energy and enthalpy of decomposition for the Fe17Sm2 nitride : The 41st annual conference on magnetism and magnetic materials (1997)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 81 (1997), S. 5109-5111 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Decomposition studies of the Fe17Sm2N3 compound were done using a Calvet-type microcalorimeter and a simultaneous gravimetric and differential thermal analyzer (DTA). The results show that the decomposition has onset temperature of 570 °C and proceeds up to 900 °C, and presents only one well determined exothermic thermal event. The calorimetric measurement shows that the enthalpy associated with the decomposition is 69 kJ/mol. The activation energy of the process was also determined using the Kissinger method applied to the DTA signal. It was observed that there was only one activation energy for the decomposition process, with a value of 339 kJ/mol. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.365152
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of Fe17R2 phases (R=Pr and Sm) oxidized at 200 °C : The 40th annual conference on magnetism and magnetic materials (1996)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 79 (1996), S. 5973-5975 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The results of oxygen absorption in temperatures ranging from 200 to 500 °C are reported for the phases Fe17R2, R=Pr and Sm. It was observed that the oxygen saturation limit is temperature dependent, being of 3 at/fu at 200 °C, 12.8 at/fu at 300 °C, 20.3 at/fu at 400 °C, and 28.5 at/fu at 500 °C. This implies that above 200 °C the phase is deeply altered by oxygen absorption. Samples with absorption at 200 °C were studied as prepared and heat treated at 500 °C/40 h. The results show that the heat-treated samples consist of Fe17R2, Fe, and R2O3. Metallographic examination, susceptibility, and Mössbauer spectroscopy data support the hypothesis that the as-prepared samples consist of a mixture of Fe17R2 and ordered interstitial Fe17R2Ox. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.362124
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  • 7
    Electronic Resource
    Electronic Resource
    A Mössbauer study of plasma nitrided iron : The 7th joint MMM-intermag conference on magnetism and magnetic materials (1998)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 83 (1998), S. 6932-6933 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Iron powder has been nitrided in a glow discharge plasma of a mixture of nitrogen and hydrogen, in order to verify the existence of a metastable iron nitrogen solid solution, previously observed from the Sm2Fe17N3 phase decomposition process. Our results show that, for short nitridation times, we obtain a mixture of Fe4N, a small amount of α-Fe and an iron nitrogen solid solution the amount of which increases after heat-treatment at 610 °C. This iron nitrogen solid solution is characterized by Mössbauer spectroscopy which shows a typical α-Fe spectrum, but with lower hyperfine field and broader linewidth. TMA measurements show that the Curie temperature is similar to that of α-Fe. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.367712
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  • 8
    Electronic Resource
    Electronic Resource
    Giant magnetoimpedance in FeAg granular alloys (2002)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 80 (2002), S. 2532-2534 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We have observed giant magnetoimpedance (GMI) in an FeAg granular alloy. Impedance and transverse susceptibility (TS) measurements as a function of the external dc magnetic field were made at room temperature. The variation of the magnetoimpedance ratio with magnetic field has been related to a corresponding TS ratio change as a function of the magnetic field. The peaks in the GMI and TS curves are in the same position and have been associated in both cases to effective anisotropy fields. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1468274
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  • 9
    Electronic Resource
    Electronic Resource
    Contact sensitivity to oak moss (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Contact dermatitis 19 (1988), S. 0 
    Details
    ISSN: 1600-0536
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Oak muss allergy was the principle allergen in contact sensitivity to perfumes (45%); 31 patients reading to oak moss were studied. The sensitivity was attributed to contact with perfumes in 20, lichens in 7 and unknown in 4. Atranorin was the most frequent allergen, followed by usnic, evernic and fumarprotocetraric acids. Concomitant allergy occurred to several lichen acids and also to balsam of Peru, colophony and other fragrance component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0536.1988.tb02951.x
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  • 10
    Electronic Resource
    Electronic Resource
    Conformational changes in cytochrome oxidase as studied by sedimentation difference
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 283 (1972), S. 181-186 
    Details
    ISSN: 0005-2728
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2728(72)90109-0
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