ISSN:
1573-5028
Keywords:
alcohol dehydrogenase 2
;
hypoxia
;
PCR
;
Petunia hybrida
;
promoter
;
transcript mapping
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The Adh2 gene from Petunia hybrida has been difficult to clone; exons 1 to 8 were isolated using PCR after unsuccessful screening of three genomic libraries. A combination of inverse and direct PCR strategies has been used to isolate upstream regions of Adh2. Here we report the cloning strategy for the nucleotide sequence of the 5′ region of Adh2 from P. hybrida, the locations of the transcriptional start site and putative TATA box, as well as comparative analyses of the upstream regions of petunia Adh2, other Adh genes and other genes induced by hypoxia.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00020123
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