ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A capillary electrophoresis method was developed for the chiral separation of D-L valine, isoleucine, and leucine. The separation of derivatized amino acids with 9-fluorenylmethyl-chloroformate was performed by micellar electrokinetic capillary chromatography. We optimized the method by varying β-cyclodextrin and sodium dodecyl sulphate concentrations in the presence of 2-propanol. The proposed method was applied to the determination of D-forms of valine, Isoleucine, and leucine in the presence of an excess of relative L forms, in commercial supplements for sport nutrition. Results demonstrated that the separation of enantiomers was possible up to an enantiomeric ratio of 1:100. The analysis of selected products confirmed the enantiomeric purity of utilized amino acids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2002.tb10287.x
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