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  • 1
    Electronic Resource
    Electronic Resource
    Purification and some properties of Papaya meleira virus, a novel virus infecting papayas in Brazil (2003)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 52 (2003), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: ‘Meleira’, or ‘sticky disease’, is currently the most damaging papaya disease in the mid-eastern Brazilian growing regions. Consistent disease transmission via latex injection, presence of similar isometric particles in the laticiferous vessels of diseased plants, and detection of double-stranded DNA in naturally and experimentally infected papaya trees suggest that a virus is the causal agent. Conclusive evidence for viral aetiology was previously lacking, mostly because every attempt to purify the putative virus from infected papayas had failed. Following the successful purification and partial characterization of the meleira virus, healthy papaya seedlings injected with purified virus particles later developed typical symptoms of the disease. Negatively stained, isometric, full and ‘empty’ purified virus particles measured 42 and 38 nm, respectively. The viral genome was a single dsRNA molecule of about 12 kbp. Several capsid proteins, ranging in size from 14·4 to 45 kDa, were consistently revealed by PAGE. Papaya meleira virus (PMeV) appears to represent a novel group of viruses, with no known similar counterpart among known plant-, vertebrate-, invertebrate- or prokaryote-infecting viruses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2003.00855.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Mycoplasma-like organisms associated with kaki decline in Brazil (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 36 (1987), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1987.tb02256.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Modelling of a Utility Boiler Using Parallel Computing (1999)
    Springer
    The journal of supercomputing 13 (1999), S. 211-232 
    Details
    ISSN: 1573-0484
    Keywords: boilers ; computational fluid dynamics ; turbulent reactive flows ; radiative heat transfer ; discrete ordinates ; parallel processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract A mathematical model for the simulation of the turbulent reactive flow and heat transfer in a power station boiler has been parallelized. The mathematical model is based on the numerical solution of the governing equations for mass, momentum, energy and transport equations for the scalar quantities. The k-ε model and the conserved scalar/prescribed probability density function formalism are employed. Radiative heat transfer is calculated using the discrete ordinates method. The code has been fully parallelized using the spatial domain decomposition approach and MPI. Calculations were performed using an IBM-SP2. It is shown that the computational requirements are reduced and the parallel efficiency increases if the mean temperature and density are calculated a priori, and stored. The role of the different parts of the code on the parallel performance is discussed. A speedup of 5.9 is achieved using 8 processors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008056714706
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  • 4
    Electronic Resource
    Electronic Resource
    Stability performance ofCynara cardunculus L. acid protease in aqueous-organic biphasic systems (1992)
    Springer
    Biotechnology letters 14 (1992), S. 179-184 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Stability performance of the acid protease ofCynara cardunculus L. in biphasic systems containing ethyl acetate,n-hexane or isooctane was investigated and compared with that of pepsin. Activity retention was higher in the system containingn-hexane. In this system 100% retention was observed up to 144 hours. Pre-saturation of phases was found to increase enzyme stability in the cases ofn-hexane and isooctane and to be an absolute requirement in the case of ethyl acetate. The results obtained suggest also that, when dealing with pre-saturated phases, log P cannot be used straightforwardly to predict enzyme stability in biphasic systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01023355
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  • 5
    Electronic Resource
    Electronic Resource
    Marituba (Bunyaviridae) virus replication in culturedAedes albopictus cells and in L-A9 cells (1986)
    Springer
    Archives of virology 90 (1986), S. 325-335 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The replication of Marituba virus (Bunyavirus genus, family Bunyaviridae), was studied inAedes albopictus (mosquito) cells. Infection ofAedes albopictus cells with Marituba virus was characterized by an initial acute phase of infection in which large amounts of virus were produced and further by a persistent phase of infection in which virus yield was much lower. No changes in host cell DNA, RNA and protein synthesis were observed inAedes albopictus cells infected with Marituba virus. In contrast in L-A9 (mouse fibroblasts) cells this virus shut-off the host macromolecular synthesis. During the replication of MTB virus in L-A9 cells three virus-specific proteins (G1, G2 and N) were detected. InAedes albopictus cells, Marituba virus replicates slowly and two virus-specific proteins (G1 and N) accumulate in these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317380
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Application of a domain decomposition technique to the mathematical modelling of a utility boiler (1993)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 36 (1993), S. 3401-3419 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Domain decomposition techniques are very effective for flow simulation, especially in domains where geometrical length scales of different orders of magnitude are present. In this work a zonal method is applied to the mathematical modelling of a power station boiler of the Portuguese Electricity Utility. The mathematical model is based on the numerical solution of the partial differential equations governing conservation of mass, momentum and energy. The zonal method is presented and the conservative treatment of the interfaces is described in detail. Emphasis is placed on the evaluation of the zonal method. The results show that the influence of the zonal method on the convergence rate of the solution algorithm is negligible. The zonal method does not influence the accuracy of the predicted results and there is continuity of the dependent variables across the interfaces. A significant reduction in CPU time is feasible due to a better distribution of grid nodes and consequent reduction in the total number of grid nodes required.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620362002
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Calculation of laminar recirculating flows using a local non-staggered grid refinement system (1991)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 12 (1991), S. 535-557 
    Details
    ISSN: 0271-2091
    Keywords: Embedding non-staggered grids ; Incompressible recirculating flows ; Finite volume method ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A grid-embedding technique for the solution of two-dimensional incompressible flows governed by the Navier-Stokes equations is presented. A finite volume method with collocated primitive variables is employed to ensure conservation at the interfaces of embedding grids as well as global conservation. The discretized equations are solved simultaneously for the whole domain, providing a strong coupling between regions of different refinement. The formulation presented herein is applicable to uniform or non-uniform Cartesian meshes. The method was applied to the solution of two scalar transport equations, to cavity flows driven by body and shear forces and to a sudden plane contraction flow. The numerical predictions are compared with the exact solutions when available and with experimental data. The results show that neither the convergence rate nor the stability of the method is affected by the presence of embedded grids. Embedded grids provide a better distribution of grid nodes over the computational domain and consequently the solution accuracy was improved. The grid-embedding technique proved also that significant savings in computing time could be achieved.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650120603
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