ISSN:
1573-5044
Keywords:
Brassica napus
;
protoplasts
;
Ficoll
;
agarose
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A method is described for regenerating callus from mesophyll protoplasts of a winter variety of Brassica napus. The method combines the use of Ficoll in an initial liquid medium, enhancing early protoplast division and cell colony formation, with a transfer to an agarose system after 10 days culture to give rapid microcalli formation. Further transfers resulted in callus regeneration and the initiation of organogenesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00034370
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