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  • 1
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary Background Chronic exposure to ultraviolet (UV) radiation induces changes in the skin structure which are mostly found in the superficial dermis and at the dermal–epidermal junction. Keratinocytes and fibroblasts contribute both to the synthesis and to the degradation of the molecules important for the integrity of this skin site. While several studies have reported on alterations of dermal components and of the functions of fibroblasts in vivo and in vitro after UV exposure, recent data suggested that keratinocytes could be the main skin cell type involved in the photoageing process. Objectives In this study, we analysed the expression of two keratinocyte molecules namely, β1 integrin (a proliferation marker) and involucrin (a differentiation marker) in sun-exposed and sun-protected facial skin of 16 healthy patients undergoing facial lifting. Methods Methods included histology, immunohistochemistry and quantitative reverse transcriptase–polymerase chain reaction analysis. Results Sun-exposed skin displayed the characteristic morphological and molecular features of dermal photoageing, compared with sun-protected skin, including dermal elastosis, diminished fibrillin and type VII collagen expression. Analysis of the epidermis in sun-exposed vs. sun-protected skin showed no histological differences, but dramatic changes in the expression of β1 integrin and involucrin. In sun-exposed skin, expression of β1 integrin protein by epidermal basal cells was reduced, paralleling a downregulation of β1 integrin mRNA, whereas involucrin protein expression was greatly enhanced in the superficial epidermal cell layers. Interestingly, the ratio between involucrin and β1 integrin protein expression was consistently increased in sun-exposed skin sites. Conclusions Collectively these results demonstrate that epidermal homeostasis is impaired by chronic UV exposure, and define β1 integrin expression as a molecular marker of the epidermal photoageing process.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  Photodamage is characterized by degradation of collagen and accumulation of abnormal elastin in the superficial dermis. Mast cells and macrophages, which are found in higher numbers in photoaged skin, have been implicated in this process.Objectives  To analyse the phenotype of haematopoietic-derived infiltrating cells in photodamaged skin.Methods  Chronically sun-exposed (preauricular) and control sun-protected (postauricular) skin was recovered from eight healthy subjects undergoing cosmetic surgery (facial lifting).Results  Histological analysis showed that sun-exposed skin harboured more infiltrating mononuclear cells than sun-protected skin. Cellular infiltrates were found at the periphery of areas of elastolysis around hair follicles in sun-exposed sites, whereas they were found in the interfollicular dermis around blood vessels and around hair follicles in sun-protected samples. Immunohistochemical analysis revealed an increased number of mast cells, macrophages and CD4+ CD45RO+ T cells in sun-exposed dermis as well as a higher number of CD1a+ dendritic cells in sun-exposed epidermis, compared with the sun-protected samples. Thus photoageing displays histological features of chronic skin inflammation. However, no molecular sign of inflammation was observed and we even found a decreased expression of interleukin-1β mRNA in sun-exposed compared with sun-protected sites. Furthermore, the patients' skin looked normal and did not display any clinical inflammation.Conclusions  Collectively, these data show that chronic ultraviolet irradiation induces alterations of innate immune cells which are recruited in sun-exposed skin without being activated.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-8248
    Keywords: Pauesia cedrobii ; Cedrobium laportei ; Cedrus atlantica ; élevage au laboratoire ; Pauesia cedrobii ; Cedrobium laportei ; Cedrus atlantica ; laboratory rearings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary The aim of this study was to attempt a permanent rearing of the parasite:Pauesia cedrobii Stary et Leclant in order to make releases in natural conditions more easily than through direct introduction. The rearings were carried out for one year and a half under 9 different climatic conditions (temperature, humidity, photoperiod) upon the specific host:Cedrobium laportei Remaudière. This aphid was multiplied on a cedar plant grown in a container, which constituted a production unit easily moved to in natural conditions. In each of the climatised rooms, with constant temperature and humidity, 2 rearing chambers were set up with fluorescent lights, one of them with 8 hours lighting, the other one with 16 hours. During the “day-time” (despite the 2 fans used for air-extraction), the chamber temperature increased by about 3°C. The parasite rearing was impossible at 4°C. On the contrary, at 8°C the rearings were possible during a certain time. Nevertheless, the temperature was still too near the lower threshold of development of the parasite, while still above that of the host. The only permanent rearings were reached at 12°C. Under these conditions we observed a good balance between the parasite, which developed normally, and its host, which never disappeared. However the experimental results show, after about one year, that the cedar plant becomes less suitable for the development of aphid colonies and for the production of parasites. At 20°C, it was impossible to maintain the parasite on its host. During all the time of the test we observed at this temperature (and also at 25°C) a decaying of the cedar plant. It seems that the upper threshold of development is lower for the parasite than for its host. Generally speaking, long days are more suitable to rearings. On the basis of these results it is possible to consider a mass rearing ofP. cedrobii to make releases in natural conditions.
    Notes: Résumé Cette étude avait pour objectif la mise au point d'un élevage du parasitePauesia cedrobii Starý & Leclant sur son hôte spécifiqueCedrobium laportei Remaudière, ce dernier étant multiplié sur des jeunes plants de cèdreCedrus atlantica Manetti. Dans des chambres climatisées à température et humidité relative constantes, on a placé des enceintes d'élevage assurant respectivement 8 et 16 heures d'éclairement. Pendant la phase d'éclairement, et malgré 2 ventilateurs chargés d'assurer l'extraction de l'air, la température des enceintes a augmenté d'environ 3°C. Au total, 9 conditions climatiques ont été testées, chacune pendant un an et demi. A 4°C, le parasite ne s'est pas multiplié. A 8°C, il s'est maintenu pendant une année environ, mais cette température s'est montrée encore trop proche de son seuil inférieur de développement et de celui de son hôte. A 12°C, on a observé un bon équilibre entre le parasite, qui s'est développé normalement, et son hôte, qui n'a jamais disparu. A 20°C, les élevages ont eu un bon rendement pendant 6 mois, mais il n'a pas été possible de les maintenir pendant plus longtemps. A cette température, et plus encore à 25°C, les plants de cèdre ont présenté des symptômes de dépérissement. A 25°C, les résultats mettent en évidence une action défavorable de la température sur tous les niveaux trophiques. En règle générale, les jours longs ont été un peu plus favorables aux élevages.
    Type of Medium: Electronic Resource
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