ISSN:
1474-8673
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Chemistry and Pharmacology
,
Medicine
Notes:
1 The aim of the study was to investigate the role of the α1D-adrenoceptor in α1-adrenoceptor-induced contraction of human prostate by means of protection experiments. 2 Responses of human prostate strips to noradrenaline were recorded, along with responses of rat aorta and vas deferens, tissues possessing predominantly α1D- and α1A-adrenoceptors respectively, for comparison. α1-adrenoceptors were then inactivated by incubation with the irreversible antagonist phenoxybenzamine. In some tissues α1A- or α1D-adrenoceptors were ‘protected’ from inactivation by incubation in the presence of the selective α1A- or 1D-adrenoceptor antagonists 5-methylurapidil and BMY 7378 before recording further responses to noradrenaline. 3 Phenoxybenzamine reduced the maximum noradrenaline-induced response and the potency of noradrenaline in all tissues. In rat vas deferens and human prostate, 5-methylurapidil protected α1A-adrenoceptors in a concentration–dependent manner. In rat aorta, 10 nm BMY 7378 almost fully protected α1D-adrenoceptors. However, concentrations of BMY 7378 up to 30-fold higher failed to protect receptors in the human prostate. 4 These results suggest that in human prostate functional α1D-adrenoceptors do not contribute to noradrenaline-induced contractile responses.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1474-8673.2002.00272.x
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