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  • 1
    Electronic Resource
    Electronic Resource
    Mouse intestinal sensitivity to cycloheximide (1971)
    Springer
    Histochemistry and cell biology 27 (1971), S. 50-62 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cycloheximide 40 mg/kg was injected into adult male mice. Biochemical, histochemical and morphological modifications of the mucosal cells of the duodenum have been recorded after one or several injections. Biochemical experiments have shown a rapid decrease of alkaline phosphatase activity in the duodenum and a significant increase in the liver. Histochemical studies have shown a total disappearance of the alkaline phosphatase activity in the Golgi zone of the absorbing cells, two hours after one injection. Acid phosphatase, thiamine-pyrophosphatase and glucose-6-phosphatase did not exhibit the same pattern. Alterations of the ultrastructure of the villus cells have been observed after several injections. Atrophy of the Golgi cisternae, appearance of autophagic vacuoles and decrease of the goblet cells secretory granules have been the most frequent modifications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263368
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Environmental stresses and strains in an extreme situation: the repair of electrometallurgy furnaces (1993)
    Springer
    International archives of occupational and environmental health 65 (1993), S. 253-258 
    Details
    ISSN: 1432-1246
    Keywords: Heat stress ; Physiological strain ; Heart rate recording ; Boilermakers ; Electrometallurgy furnace
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Whenever a continuous casting furnace breaks down, the emergency intervention necessary to repair it has to be carried out under exceptional environmental conditions caused mainly by heat, as the furnace must be stopped for the shortest possible time. In this study, we aimed to evaluate the stresses and strains to which boilermakers are subjected during the replacement of an electrode element of a 20 MW furnace. The thermal stress was evaluated by the wet bulb globe temperature (WBGT) index. CO2 was measured continuously at the furnace periphery and sporadically in the center of the furnace using an electrochemical method, while CO was also measured in both areas, using Dräger tubes. Dusts were sampled by a CPM3 (Andersen particle fractionating sampler) and a CIP10 (personal sampler). The strain was evaluated by continuous ECG recording with an Aclan IFC 85, breathing performance was assessed with an HI 298 microspirometer, and blood oxygen saturation was evaluated using a Biox oximeter. Thermal stresses are extreme: WBGT was 55°C in the furnace center and 34°C in the furnace periphery. In spite of the ventilation, the reduction in heat during the 6 h of the intervention was negligible and did not provide sufficient cooling. The analysis of gases and dusts were of minor interest, although the mean CO level at the furnace periphery was 40 ppm, with a peak level of 140 ppm in furnace center. CO2 and SO2 levels did not exceed TLV-TWA and TLV-Stel values. The damage to the human body was significant with average heart rates of 150/min during the working period and a “relative cardiac cost” of 37%–69%, leading to major fatigue. The thermal strain was the most stressful (“thermally induced additional heart beats” represented 86.8%–100% of the global cardiac cost). Spirometers did not revealed a reduction in dynamic lung volumes after 4 hours' work. The SaO2 and HbCO were not significantly modified. The overall difficulty of this time-consuming repair and the risks inherent in the physical and thermal stresses placed on the human body show that urgent technical improvements are badly needed; this is especially true of the tools used for manutention, the organization and duration of the repair, the planning of rest periods, and medical supervision.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00381199
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Establishment of a rat Sertoli cell line that displays the morphological and some of the functional characteristics of the native cell (1997)
    Springer
    Cell biology and toxicology 13 (1997), S. 453-463 
    Details
    ISSN: 1573-6822
    Keywords: apoptosis ; extracellular matrix ; phagocytosis ; Sertoli cells ; SV40-T
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Primary rat Sertoli cells are widely used as a model for mechanistic and toxicological studies, since they are often the target of toxicants in vivo. However, their isolation from testicular homogenates is tedious and requires the regular use of numerous immature animals. It is therefore of great interest to have available established cell lines that are usable in vitro for unlimited periods and closely similar to native cells. To this end, we have established a line of Wistar rat Sertoli cells (SerW3) by immortalization of fresh primary cells with the T antigens of the Simian virus (SV40). When plated on Matrigel, this cell line presents many of the functional characteristics of Sertoli cells in vivo. In addition, they are sensitive to cisplatin and secrete transferrin, although they do not show a clear response to follicle-stimulating hormone. They also present many morphological similarities, including the presence of tight junctions which mimic the natural epithelial barrier. Like Sertoli cells in vivo, they show extensive phagocytic activity. Finally, they display all the characteristics of immortalized, but not transformed, cells, i.e., topo-inhibition and apoptosis at confluence or under serum deprivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007475928452
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    Paper (German National Licenses)
    Fulltext
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